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Journal of Lipid Research, Vol. 43, 1086-1095, July 2002
Copyright © 2002 by Lipid Research, Inc.
* Human Nutrition Research Center, United States Department of Agriculture, Beltsville, Maryland 20705
Departments of Anatomy & Cell Biology and Pediatrics, SUNY Downstate Medical Center, Brooklyn, New York 11203
Department of Pharmacology, University of the Sciences in Philadelphia, Philadelphia, Pennsylvania 19404
2 To whom correspondence should be addressed. e-mail: during{at}bhnrc.arsusda.gov
In presence of oleate and taurocholate, differentiated CaCo-2 cell monolayers on membranes were able to assemble and secrete chylomicrons. Under these conditions, both cellular uptake and secretion into chylomicrons of ß-carotene (ß-C) were curvilinear, time-dependent (2–16 h), saturable, and concentration-dependent (apparent Km of 7–10 µM) processes. Under linear concentration conditions at 16 h incubation, the extent of absorption of all-trans ß-C was 11% (80% in chylomicrons), while those of 9-cis- and 13-cis-ß-C were significantly lower (2–3%). The preferential uptake of the all-trans isomer was also shown in hepatic stellate HSC-T6 cells and in a cell-free system from rat liver (microsomes), but not in endothelial EAHY cells or U937 monocyte-macrophages. Moreover, extents of absorption of 
-carotene (-C), lutein (LUT), and lycopene (LYC) in CaCo-2 cells were 10%, 7%, and 2.5%, respectively. Marked carotenoid interactions were observed between LYC/ß-C and ß-C/-C.
The present results indicate that ß-C conformation plays a major role in its intestinal absorption and that cis isomer discrimination is at the levels of cellular uptake and incorporation into chylomicrons. Moreover, the kinetics of cellular uptake and secretion of ß-C, the inhibition of the intestinal absorption of one carotenoid by another, and the cellular specificity of isomer discrimination all suggest that carotenoid uptake by intestinal cells is a facilitated process.
Abbreviations: -C, -carotene; ß-C, ß-carotene; CM, chylomicrons; LUT, lutein; LYC, lycopene; NEAA, non essential amino acids; OA, oleic acid; PL, phospholipids; TC, taurocholate
Supplementary key words cis isomers • -carotene • lycopene • lutein • chylomicrons • human intestinal model
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