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Journal of Lipid Research, Vol. 43, 1303-1311, August 2002
Copyright © 2002 by Lipid Research, Inc.

Regulation of MTP expression in developing swine

Song Lu^*, Mark Huffman^*, Ying Yao^*, Charles M. Mansbach, II^,, Xiangying Cheng^*, Songmai Meng^* and Dennis D. Black^1,*

^* Children's Foundation Research Center of Memphis at Le Bonheur Children's Medical Center, Memphis, TN 38103
Department of Medicine, University of Tennessee Health Science Center, Memphis, TN 38103
The Veterans Affairs Medical Center, Memphis, TN 38104

¹ To whom correspondence should be addressed. e-mail: dblack{at}utmem.edu

To define the developmental expression of microsomal triglyceride transfer protein (MTP) large subunit mRNA and protein, samples of small intestine and liver were collected from 40-day gestation fetal, 2-day-old newborn, 3-week-old suckling, and 2-month-old weanling swine. In fetal animals, MTP mRNA expression was high in intestine and liver. Postnatally, jejunal expression paralleled the intake of a high-fat breast milk diet and declined after weaning. Ileal expression was comparable with that of jejunum in 2-day-old animals, but declined to low levels afterward. Hepatic expression declined postnatally and remained low. MTP protein expression generally paralleled mRNA expression, except in fetal intestine in which no 97 kDa protein was detected. In 2-day-old piglets, a high-triacylglycerol diet increased jejunal and ileal MTP mRNA levels, as compared to a low-triacylglycerol diet. To test the roles of glucocorticoids and fatty acids in MTP regulation, a newborn swine enterocyte cell line (IPEC-1) was used. Except at day 2 of differentiation, dexamethasone did not influence MTP expression. Fatty acids either up-regulated or down-regulated MTP expression, depending on the specific fatty acid and duration of exposure.

Although programmed genetic cues regulate MTP expression during development, clearly the amount and fatty acid composition of dietary lipid also play regulatory roles.

Abbreviations: ER, endoplasmic reticulum; GM, growth medium; MTP, microsomal triglyceride transfer protein; PDI, protein disulfide isomerase; RACE, rapid amplification of cDNA ends; RQ1, RNA Qualified 1

Supplementary key words dexamethasone • eicosapentaenoic acid • liver • microsomal triglyceride transfer protein • oleic acid • reverse transcriptase-polymerase chain reaction • small intestine • stearic acid • Western blot

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