HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lu, S. Articles by Black, D. D. Search for Related Content PubMed PubMed Citation Articles by Lu, S. Articles by Black, D. D. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 43, 1303-1311, August 2002
Copyright © 2002 by Lipid Research, Inc.

Regulation of MTP expression in developing swine

Song Lu^*, Mark Huffman^*, Ying Yao^*, Charles M. Mansbach, II^,, Xiangying Cheng^*, Songmai Meng^* and Dennis D. Black^1,*

^* Children's Foundation Research Center of Memphis at Le Bonheur Children's Medical Center, Memphis, TN 38103
Department of Medicine, University of Tennessee Health Science Center, Memphis, TN 38103
The Veterans Affairs Medical Center, Memphis, TN 38104

¹ To whom correspondence should be addressed. e-mail: dblack{at}utmem.edu

To define the developmental expression of microsomal triglyceride transfer protein (MTP) large subunit mRNA and protein, samples of small intestine and liver were collected from 40-day gestation fetal, 2-day-old newborn, 3-week-old suckling, and 2-month-old weanling swine. In fetal animals, MTP mRNA expression was high in intestine and liver. Postnatally, jejunal expression paralleled the intake of a high-fat breast milk diet and declined after weaning. Ileal expression was comparable with that of jejunum in 2-day-old animals, but declined to low levels afterward. Hepatic expression declined postnatally and remained low. MTP protein expression generally paralleled mRNA expression, except in fetal intestine in which no 97 kDa protein was detected. In 2-day-old piglets, a high-triacylglycerol diet increased jejunal and ileal MTP mRNA levels, as compared to a low-triacylglycerol diet. To test the roles of glucocorticoids and fatty acids in MTP regulation, a newborn swine enterocyte cell line (IPEC-1) was used. Except at day 2 of differentiation, dexamethasone did not influence MTP expression. Fatty acids either up-regulated or down-regulated MTP expression, depending on the specific fatty acid and duration of exposure.

Although programmed genetic cues regulate MTP expression during development, clearly the amount and fatty acid composition of dietary lipid also play regulatory roles.

Abbreviations: ER, endoplasmic reticulum; GM, growth medium; MTP, microsomal triglyceride transfer protein; PDI, protein disulfide isomerase; RACE, rapid amplification of cDNA ends; RQ1, RNA Qualified 1

Supplementary key words dexamethasone • eicosapentaenoic acid • liver • microsomal triglyceride transfer protein • oleic acid • reverse transcriptase-polymerase chain reaction • small intestine • stearic acid • Western blot

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Xie, J. Luo, S. Kennedy, and N. O. Davidson Conditional Intestinal Lipotoxicity in Apobec-1-/- Mttp-IKO Mice: A SURVIVAL ADVANTAGE FOR MAMMALIAN INTESTINAL APOLIPOPROTEIN B mRNA EDITING J. Biol. Chem., November 9, 2007; 282(45): 33043 - 33051. [Abstract] [Full Text] [PDF]

				D. D. Black Development and Physiological Regulation of Intestinal Lipid Absorption. I. Development of intestinal lipid absorption: cellular events in chylomicron assembly and secretion Am J Physiol Gastrointest Liver Physiol, September 1, 2007; 293(3): G519 - G524. [Abstract] [Full Text] [PDF]

				X. Pan and M. M. Hussain Diurnal Regulation of Microsomal Triglyceride Transfer Protein and Plasma Lipid Levels J. Biol. Chem., August 24, 2007; 282(34): 24707 - 24719. [Abstract] [Full Text] [PDF]

				S. Leng, S. Lu, Y. Yao, Z. Kan, G. S. Morris, B. R. Stair, M. A. Cherny, and D. D. Black Hepatocyte nuclear factor-4 mediates apolipoprotein A-IV transcriptional regulation by fatty acid in newborn swine enterocytes Am J Physiol Gastrointest Liver Physiol, August 1, 2007; 293(2): G475 - G483. [Abstract] [Full Text] [PDF]

				C.-M. Li, J. B. Presley, X. Zhang, N. Dashti, B. H. Chung, N. E. Medeiros, C. Guidry, and C. A. Curcio Retina expresses microsomal triglyceride transfer protein: implications for age-related maculopathy J. Lipid Res., April 1, 2005; 46(4): 628 - 640. [Abstract] [Full Text] [PDF]

				V. Carriere, R. Vidal, K. Lazou, M. Lacasa, F. Delers, A. Ribeiro, M. Rousset, J. Chambaz, and J. M. Lacorte HNF-4-dependent Induction of Apolipoprotein A-IV Gene Transcription by an Apical Supply of Lipid Micelles in Intestinal Cells J. Biol. Chem., February 18, 2005; 280(7): 5406 - 5413. [Abstract] [Full Text] [PDF]