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Journal of Lipid Research, Vol. 43, 1335-1340, August 2002
Copyright © 2002 by Lipid Research, Inc.

Methods

Recovery of gangliosides from aqueous solutions on styrene-divinylbenzene copolymer columns¹

Iuliana Popa^*,, Cristina Vlad, Jacques Bodennec^* and Jacques Portoukalian^2,*

^* Laboratory of Dermatology, INSERM U.346, Hospital Edouard Herriot 69437 Lyon Cx 03, France
Institute of Macromolecular Chemistry Petru Poni, Aleea Gr. Ghica Voda 41A, Iassy 6600, Romania

² To whom correspondence should be addressed. e-mail: portoukalian{at}lyon.inserm.fr

An investigation was made using a styrene-divinylbenzene copolymer as a solid phase sorbent to recover gangliosides from aqueous solutions. A comparison with octadecyl-bonded (C18) silica gel showed that the general procedure used to purify gangliosides on C18 silica gel could be used with the copolymer. The yield of gangliosides depended on various parameters such as the composition of the conditioning solution, the salt concentration of the loading solution, and the amount of applied gangliosides per gram of copolymer. In optimal conditions, the recovery of gangliosides and other lipids present in the upper phases of partition was higher than 95%. Using radiolabeled gangliosides, it was found that gangliosides present in serum-containing medium could also be quantitatively recovered on copolymer, provided the medium was diluted with an equal volume of methanol prior to its application onto the column.

The major advantage of the copolymer is its high stability in acidic or alkaline conditions that allows multiple cycles of cleaning and reconditioning of the sorbent without alteration of its chromatographic properties.

Abbreviations: C18 silica gel, octadecylsilyl-bonded silica gel; SPE, solid phase extraction; TUP, theoretical upper phase of partition

Supplementary key words chromatography • isolation • solid phase extraction

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