HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M200292-JLR200v1 44/2/380    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Balestrieri, M. L. Articles by Servillo, L. Search for Related Content PubMed PubMed Citation Articles by Balestrieri, M. L. Articles by Servillo, L. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 44, 380-387, February 2003
Copyright © 2003 by Lipid Research, Inc.

Modulation by flavonoids of PAF and related phospholipids in endothelial cells during oxidative stress

Maria Luisa Balestrieri^*, Domenico Castaldo, Ciro Balestrieri^*, Lucio Quagliuolo^*, Alfonso Giovane^* and Luigi Servillo^1,*

^* Department of Biochemistry and Biophysics, Second University of Naples, Italy
Stazione Sperimentale Industria Essenze e Derivati Agrumari, Reggio Calabria, Italy

¹ To whom correspondence should be addressed. e-mail: luigi.servillo{at}unina2.it

PAF-dependent transacetylase (TA) modifies the functions of platelet-activating factor (PAF), a potent inflammatory lipid, either by transferring the acetyl group from PAF to lysophospholipids (TA_L activity), or to sphingosine (TA_S activity) or by hydrolyzing PAF (acetylhydrolase activity). In stimulated endothelial cells (EC), TA_L activity contributes to the synthesis of acyl-PAF, an acyl analog of PAF, that antagonizes PAF functions and is regulated by the cellular redox state. In this study, we investigated the possible involvement of TA in the flavonoid antioxidant mechanism(s) during oxidative stress in EC induced by hydrogen peroxide. The treatment of EC with H₂O₂ resulted in 4-fold increase of the acetyl-CoA acetyltransferase activity (AT), that is responsible for PAF biosynthesis, while the TA_L activity increased only by 53%. However, the preincubation of H₂O₂-treated EC with the flavonoids hesperedin, naringin, and quercetin strongly inhibited AT activity and activated TA_L by 290%, 340%, and 250%, respectively.

The induction of TA_L activity resulted in enhanced biosynthesis of 1-acyl-2-[³H]acetyl-PAF in intact EC and was related to the flavonoid structure. These findings suggest that TA_L is involved in the flavonoid anti-inflammatory action by enhancing the production of acyl-PAF.

Abbreviations: AH, acetylhydrolase; AT, acetyltransferase activity; CP, creatine phosphate; CPK, creatine phosphokinase; EC, endothelial cell; LPE, lyso-glycerophosphoethanolamine; PAF, platelet-activating factor; PLA₂, phospholipase A₂; PLC, phospholipase C; TA, transacetylase; TA_L, transacetylase lysophospholipids; TA_S, transacetylase sphingosine

Supplementary key words atherosclerosis • inflammation • transacetylase lysophospholipids • platelet-activating factor

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. Servillo, C. Balestrieri, A. Giovane, P. Pari, D. Palma, G. Giannattasio, M. Triggiani, and M. L. Balestrieri Lysophospholipid Transacetylase in the Regulation of Paf Levels in Human Monocytes and Macrophages FASEB J, May 1, 2006; 20(7): 1015 - 1017. [Abstract] [Full Text] [PDF]