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Journal of Lipid Research, Vol. 44, 450-454, March 2003
Copyright © 2003 by Lipid Research, Inc.

Rapid Communication

Conversion of hexadecanoic acid to hexadecenoic acid by rat 6-desaturase

Hervé Guillou^*, Vincent Rioux^*, Daniel Catheline^*, Jean-Noël Thibault, Monique Bouriel^*, Sophie Jan^*,, Sabine D'Andrea^* and Philippe Legrand^1,*

^* Laboratoire de Biochimie, INRA-ENSA, Rennes, France
Université du Maine, Le Mans, France
UMR-VP INRA-ENSA, Rennes, France

¹ To whom correspondence should be addressed. e-mail: philippe.legrand{at}agrorennes.educagri.fr

ABSTRACT

A higher content of C16:1 n-10 has recently been reported in the preputial gland of mice with a targeted disruption of the gene encoding stearoyl-CoA desaturase 1 (SCD1^-/- mice) when compared with wild-type mice. This result has provided the first physiological evidence for the presence and regulation of a palmitoyl-CoA 6-desaturase in mammals. To investigate the putative involvement of the known 6-desaturase (FADS2) in this process, COS-7 cells expressing rat 6-desaturase were incubated with C16:0. Transfected cells were able to synthesize C16:1 n-10, while nontransfected cells did not produce any C16:1 n-10.

Evidence is therefore presented that the rat 6-desaturase, which acts on the 18- and 24-carbon fatty acids of the n-6 and n-3 series, is also able to catalyze palmitic acid 6-desaturation.

Abbreviations: FCS, fetal calf serum; GC, gas chromatography; MS, mass spectrometry; pCMV/6, rat 6-desaturase open reading frame inserted in pCMV vector; pCDNA3/9, rat 9-desaturase open reading frame inserted in pcDNA3 vector

Supplementary key words desaturation • saturated fatty acids • FADS2 • animal

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