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Journal of Lipid Research, Vol. 44, 554-559, March 2003
Copyright © 2003 by Lipid Research, Inc.

Separate myocardial ethanolamine phosphotransferase activities responsible for plasmenylethanolamine and phosphatidylethanolamine synthesis

David A. Ford¹

Department of Biochemistry and Molecular Biology, St. Louis University Health Sciences Center, St. Louis, MO 63104

¹ To whom correspondence should be addressed. e-mail: fordda{at}slu.edu

Ethanolamine phosphotransferase (EPT) is a key enzyme responsible for the synthesis of ethanolamine glycerophospholipids. Plasmenylethanolamine is a predominant molecular subclass of ethanolamine glycerophospholipids in the heart. The present study was designed to identify the selective use of 1-O-alk-1'-enyl-2-acyl-sn-glycerol as a substrate for EPT as a mechanism responsible for the predominance of plasmenylethanolamine in the rabbit heart. EPT activity in rabbit myocardial membranes using 1,2-diacyl-sn-glycerol as substrate is activated by Mn²⁺, inhibited by dithiobisnitrobenzoic acid (DTNB) and is unaffected by Ca²⁺. In contrast, ethanolamine phosphotransferase activity using 1-O-alk-1'-enyl-2-acyl-sn-glycerol as substrate is inhibited by Mn²⁺ and Ca²⁺, but is activated by DTNB. Additionally, ethanolamine phosphotransferase activity using 1-O-alk-1'-enyl-2-acyl-sn-glycerol substrate was more sensitive to thermal denaturation compared with that of 1,2-diacyl-sn-glycerol.

Taken together, these results suggest that separate ethanolamine phosphotransferase activities are present in heart membranes that are responsible for the synthesis of phosphatidylethanolamine and plasmenylethanolamine.

Abbreviations: AAG, 1-O-alk-1'-enyl-2-acyl-sn-glycerol; CGP, choline glycerophospholipid; CPT, choline phosphotransferase; DAG, 1,2-diacyl-sn-glycerol; EGP, ethanolamine glycerophospholipid; EPT, ethanolamine phosphotransferase; GPC, sn-glycero-3-phosphocholine; 16:0-20:4, 1-hexadecanoyl-2-eicosatetra-5',8',11',14'-enoyl; a16:0-20:4, 1-O-hexadec-1'-enyl-2-eicosatetra-5',8',11',14'-enoyl

Supplementary key words myocardium • ethanolamine glycerophospholipids • choline phosphotransferase

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