J. Lipid Res.
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Originally published In Press as doi:10.1194/jlr.M200426-JLR200 on December 16, 2002

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Journal of Lipid Research, Vol. 44, 554-559, March 2003
Copyright © 2003 by Lipid Research, Inc.

Separate myocardial ethanolamine phosphotransferase activities responsible for plasmenylethanolamine and phosphatidylethanolamine synthesis

David A. Ford1

Department of Biochemistry and Molecular Biology, St. Louis University Health Sciences Center, St. Louis, MO 63104

1 To whom correspondence should be addressed. e-mail: fordda{at}slu.edu

Ethanolamine phosphotransferase (EPT) is a key enzyme responsible for the synthesis of ethanolamine glycerophospholipids. Plasmenylethanolamine is a predominant molecular subclass of ethanolamine glycerophospholipids in the heart. The present study was designed to identify the selective use of 1-O-alk-1'-enyl-2-acyl-sn-glycerol as a substrate for EPT as a mechanism responsible for the predominance of plasmenylethanolamine in the rabbit heart. EPT activity in rabbit myocardial membranes using 1,2-diacyl-sn-glycerol as substrate is activated by Mn2+, inhibited by dithiobisnitrobenzoic acid (DTNB) and is unaffected by Ca2+. In contrast, ethanolamine phosphotransferase activity using 1-O-alk-1'-enyl-2-acyl-sn-glycerol as substrate is inhibited by Mn2+ and Ca2+, but is activated by DTNB. Additionally, ethanolamine phosphotransferase activity using 1-O-alk-1'-enyl-2-acyl-sn-glycerol substrate was more sensitive to thermal denaturation compared with that of 1,2-diacyl-sn-glycerol.

Taken together, these results suggest that separate ethanolamine phosphotransferase activities are present in heart membranes that are responsible for the synthesis of phosphatidylethanolamine and plasmenylethanolamine.

Abbreviations: AAG, 1-O-alk-1'-enyl-2-acyl-sn-glycerol; CGP, choline glycerophospholipid; CPT, choline phosphotransferase; DAG, 1,2-diacyl-sn-glycerol; EGP, ethanolamine glycerophospholipid; EPT, ethanolamine phosphotransferase; GPC, sn-glycero-3-phosphocholine; 16:0-20:4, 1-hexadecanoyl-2-eicosatetra-5',8',11',14'-enoyl; a16:0-20:4, 1-O-hexadec-1'-enyl-2-eicosatetra-5',8',11',14'-enoyl

Supplementary key words myocardium • ethanolamine glycerophospholipids • choline phosphotransferase


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