HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M200380-JLR200v1 44/3/621    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yu, S.-H. Articles by Possmayer, F. Search for Related Content PubMed PubMed Citation Articles by Yu, S.-H. Articles by Possmayer, F. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 44, 621-629, March 2003
Copyright © 2003 by Lipid Research, Inc.

Lipid compositional analysis of pulmonary surfactant monolayers and monolayer-associated reservoirs

Shou-Hwa Yu and Fred Possmayer¹

Departments of Obstetrics & Gynaecology and Biochemistry, CIHR Group in Fetal and Neonatal Health and Development, The University of Western Ontario, London, ON, Canada N6A 5A5

¹ To whom correspondence should be addressed. e-mail: fpossmay{at}uwo.ca

Pulmonary surfactant is a lipid:protein complex containing dipalmitoyl-phosphatidylcholine (DPPC) as the major component. Recent studies indicate adsorbed surfactant films consist of a surface monolayer and a monolayer-associated reservoir. It has been hypothesized that the monolayer and its functionally contiguous reservoir may be enriched in DPPC relative to bulk phase surfactant. We investigated the compositional relationship between the monolayer and its reservoir using paper-supported wet bridges to transfer films from adsorbing dishes to clean surfaces on spreading dishes. Spreading films appear to form monolayers in the spreading dishes. We employed bovine lipid extract surfactant [BLES(chol)] containing [³H]DPPC and either [¹⁴C]palmitoyl, oleoyl-phosphatidylcholine (POPC), [¹⁴C]dipalmitoyl-phosphatidylglycerol (DPPG), [¹⁴C]palmitoyl, oleoyl-phosphatidylglycerol (POPG), or [14C]cholesterol. Radiolabeled phosphatidylglycerols were prepared using phospholipase D. The studies demonstrated that the [³H]DPPC-[¹⁴C] POPC ratios were the same in the prepared BLES dispersions as in Langmuir-Blodgett films, indicating a lack of DPPC selectivity during film formation. Furthermore, identical ³H-¹⁴C isotopic ratios were observed with DPPC and either ¹⁴C-labeled POPC, DPPG, POPG, or cholesterol in the original dispersions, the bulk phases in adsorption dish D1, and monolayers recovered from spreading dish D2. These relationships remained unperturbed with 2-fold increases in bulk concentrations in D1 and 10-fold variations in D1-D2 surface area.

These results indicate adsorbed surfactant monolayers and their associated reservoirs possess similar lipid compositions and argue against selective adsorption of DPPC.

Supplementary key words bovine lipid extract surfactant • cholesterol • dipalmitoylphosphatidylcholine • Langmuir-Blodgett films • phosphatidylcholine • phosphatidylglycerol • respiratory distress syndrome • surfactant multilayers

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Y. Zuo and F. Possmayer How does pulmonary surfactant reduce surface tension to very low values? J Appl Physiol, May 1, 2007; 102(5): 1733 - 1734. [Full Text] [PDF]

				H. Nakanishi, H. Shindou, D. Hishikawa, T. Harayama, R. Ogasawara, A. Suwabe, R. Taguchi, and T. Shimizu Cloning and Characterization of Mouse Lung-type Acyl-CoA:Lysophosphatidylcholine Acyltransferase 1 (LPCAT1): EXPRESSION IN ALVEOLAR TYPE II CELLS AND POSSIBLE INVOLVEMENT IN SURFACTANT PRODUCTION J. Biol. Chem., July 21, 2006; 281(29): 20140 - 20147. [Abstract] [Full Text] [PDF]

				J. Bernardino de la Serna, J. Perez-Gil, A. C. Simonsen, and L. A. Bagatolli Cholesterol Rules: DIRECT OBSERVATION OF THE COEXISTENCE OF TWO FLUID PHASES IN NATIVE PULMONARY SURFACTANT MEMBRANES AT PHYSIOLOGICAL TEMPERATURES J. Biol. Chem., September 24, 2004; 279(39): 40715 - 40722. [Abstract] [Full Text] [PDF]

				A. Frisk, J. R. Schurr, G. Wang, D. C. Bertucci, L. Marrero, S. H. Hwang, D. J. Hassett, and M. J. Schurr Transcriptome Analysis of Pseudomonas aeruginosa after Interaction with Human Airway Epithelial Cells Infect. Immun., September 1, 2004; 72(9): 5433 - 5438. [Abstract] [Full Text] [PDF]

				A. K. Panda, K. Nag, R. R. Harbottle, K. Rodriguez-Capote, R. A. W. Veldhuizen, N. O. Petersen, and F. Possmayer Effect of Acute Lung Injury on Structure and Function of Pulmonary Surfactant Films Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 641 - 650. [Abstract] [Full Text] [PDF]