Advertisement
J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1194/jlr.M200449-JLR200 on January 16, 2003

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M200449-JLR200v1
44/4/807    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, L.
Right arrow Articles by Brown, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, L.
Right arrow Articles by Brown, D. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 44, 807-815, April 2003
Copyright © 2003 by Lipid Research, Inc.

Caveolin-1 does not affect SR-BI-mediated cholesterol efflux or selective uptake of cholesteryl ester in two cell lines

Libo Wang1,*, Margery A. Connelly{dagger}, Anne G. Ostermeyer*, Hsu-hsin Chen2,*, David L. Williams{dagger} and Deborah A. Brown3,*

* Departments of Biochemistry and Cell Biology, SUNY at Stony Brook, Stony Brook NY, 11794
{dagger} Pharmacological Sciences, SUNY at Stony Brook, Stony Brook NY, 11794

3 To whom correspondence should be addressed. e-mail: deborah.brown{at}sunysb.edu

Free cholesterol (FC) has been reported to efflux from cells through caveolae, which are 50–100 nm plasma membrane pits. The 22 kDa protein caveolin-1 is concentrated in caveolae and is required for their formation. The HDL scavenger receptor BI (SR-BI), which stimulates both FC efflux and selective uptake of HDL-derived cholesteryl ester (CE), has been reported to be concentrated in caveolae, suggesting that this localization facilitates flux of FC and CE across the membrane. However, we found that overexpression of caveolin-1 in Fischer rat thyroid (FRT) cells, which lack caveolin-1 and caveolae, or HEK 293 cells, which normally express very low levels of caveolin-1, did not affect FC efflux to HDL or liposomes. Transient expression of SR-B1 did not affect this result. Similarly, caveolin-1 expression did not affect selective uptake of CE from labeled HDL particles in FRT or HEK 293 cells transfected with SR-BI.

We conclude that basal and SR-BI-stimulated FC efflux to HDL and liposomes and SR-BI-mediated selective uptake of HDL CE are not affected by caveolin-1 expression in HEK 293 or FRT cells.

Abbreviations: CE, cholesteryl ester; DOPC, dioleoyl phosphatidylcholine; FC, free cholesterol; FRT, Fischer rat thyroid; RCT, reverse cholesterol transport; SR-BI, scavenger receptor BI

Supplementary key words caveolae • cholesterol traffic • lipid raft • scavenger receptor BI • high density lipoprotein


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
V. Y. Fujimoto, J. P. Kane, B. Y. Ishida, M. S. Bloom, and R. W. Browne
High-density lipoprotein metabolism and the human embryo
Hum. Reprod. Update, January 1, 2010; 16(1): 20 - 38.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. G. Frank, S. Pavlides, M. W.-C. Cheung, K. Daumer, and M. P. Lisanti
Role of caveolin-1 in the regulation of lipoprotein metabolism
Am J Physiol Cell Physiol, July 1, 2008; 295(1): C242 - C248.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Sengupta, F. Philip, and S. Scarlata
Caveolin-1 alters Ca2+ signal duration through specific interaction with the G{alpha}q family of G proteins
J. Cell Sci., May 1, 2008; 121(9): 1363 - 1372.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S.-K. Cha, T. Wu, and C.-L. Huang
Protein kinase C inhibits caveolae-mediated endocytosis of TRPV5
Am J Physiol Renal Physiol, May 1, 2008; 294(5): F1212 - F1221.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
Y.-C. Lin, C. Ma, W.-C. Hsu, H.-F. Lo, and V. C. Yang
Molecular interaction between caveolin-1 and ABCA1 on high-density lipoprotein-mediated cholesterol efflux in aortic endothelial cells
Cardiovasc Res, August 1, 2007; 75(3): 575 - 583.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
X. Wang, H. Mu, H. Chai, D. Liao, Q. Yao, and C. Chen
Human Immunodeficiency Virus Protease Inhibitor Ritonavir Inhibits Cholesterol Efflux from Human Macrophage-Derived Foam Cells
Am. J. Pathol., July 1, 2007; 171(1): 304 - 314.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P. G. Frank, M. W.-C. Cheung, S. Pavlides, G. Llaverias, D. S. Park, and M. P. Lisanti
Caveolin-1 and regulation of cellular cholesterol homeostasis
Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H677 - H686.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
C. J. Harder, G. Vassiliou, H. M. McBride, and R. McPherson
Hepatic SR-BI-mediated cholesteryl ester selective uptake occurs with unaltered efficiency in the absence of cellular energy
J. Lipid Res., March 1, 2006; 47(3): 492 - 503.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Wharton, T. Meshulamy, G. Vallega, and P. Pilch
Dissociation of Insulin Receptor Expression and Signaling from Caveolin-1 Expression
J. Biol. Chem., April 8, 2005; 280(14): 13483 - 13486.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Fu, A. Hoang, G. Escher, R. G. Parton, Z. Krozowski, and D. Sviridov
Expression of Caveolin-1 Enhances Cholesterol Efflux in Hepatic Cells
J. Biol. Chem., April 2, 2004; 279(14): 14140 - 14146.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Levy, D. Menard, I. Suc, E. Delvin, V. Marcil, L. Brissette, L. Thibault, and M. Bendayan
Ontogeny, immunolocalisation, distribution and function of SR-BI in the human intestine
J. Cell Sci., January 15, 2004; 117(2): 327 - 337.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Peng, W. Akmentin, M. A. Connelly, S. Lund-Katz, M. C. Phillips, and D. L. Williams
Scavenger Receptor BI (SR-BI) Clustered on Microvillar Extensions Suggests that This Plasma Membrane Domain Is a Way Station for Cholesterol Trafficking between Cells and High-Density Lipoprotein
Mol. Biol. Cell, January 1, 2004; 15(1): 384 - 396.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
K. E. Schmid, W. S. Davidson, L. Myatt, and L. A. Woollett
Transport of cholesterol across a BeWo cell monolayer: implications for net transport of sterol from maternal to fetal circulation
J. Lipid Res., October 1, 2003; 44(10): 1909 - 1918.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
B. L. Trigatti, M. Krieger, and A. Rigotti
Influence of the HDL Receptor SR-BI on Lipoprotein Metabolism and Atherosclerosis
Arterioscler Thromb Vasc Biol, October 1, 2003; 23(10): 1732 - 1738.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
A. Rigotti, H. E. Miettinen, and M. Krieger
The Role of the High-Density Lipoprotein Receptor SR-BI in the Lipid Metabolism of Endocrine and Other Tissues
Endocr. Rev., June 1, 2003; 24(3): 357 - 387.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement