| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* INSERM U 499 and GENALYS, Faculté RTH Laennec, 69008, Lyon, France
School of Dietetics and Human Nutrition, McGill University, Montreal, Quebec
1 To whom correspondence should be addressed. e-mail: beylot{at}laennec.univ-lyon1.fr
We assessed the contributions of human liver and adipose tissue de novo lipogenesis (DNL) to triacylglycerol (TAG) synthesis. Volunteers were fed a high-energy, high-carbohydrate diet (HC, n = 5) or a normocaloric diet (NC, n = 10). NC subjects remained in the fasting state (Study 1, n = 5) or received oral glucose (Study 2, n = 5) throughout the test (12 h). HC subjects remained in the fasting state (Study 3). They ingested deuterated water and [U-13C]acetate to trace lipogenesis. Adipose tissue fatty-acid (FA) synthase (FAS), acetyl-CoA carboxylase 1 (ACC1), and SREBP-1c mRNA were measured. Plasma TAG-FA was labeled by 13C and deuterium showing active liver lipogenesis, which was stimulated (P < 0.05) by oral glucose and HC diet. Adipose tissue TAG had no detectable 13C enrichment in any test, showing no significant incorporation of TAG-FA provided by liver lipogenesis, but were labeled by deuterium in all tests, showing active DNL in situ; however, rough quantitative estimates showed that adipose DNL was minimal (<1 g), and poorly stimulated by oral glucose or HC diet. mRNA levels were not increased by the HC diet.
Adipose DNL is active in humans, but contributes little to TAG stores and is less responsive than liver DNL to stimulation by carbohydrates.
Supplementary key words stable isotopes • mRNA • obesity • sterol-regulatory element-binding protein-1c • fatty-acid synthase • acetyl-CoA carboxylase
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Qi and B. Rodrigues Glucocorticoids produce whole body insulin resistance with changes in cardiac metabolism Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E654 - E667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Murphy Stable isotope methods for the in vivo measurement of lipogenesis and triglyceride metabolism J Anim Sci, April 1, 2006; 84(13_suppl): E94 - E. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lundgren, J. Buren, T. Ruge, T. Myrnas, and J. W. Eriksson Glucocorticoids Down-Regulate Glucose Uptake Capacity and Insulin-Signaling Proteins in Omental But Not Subcutaneous Human Adipocytes J. Clin. Endocrinol. Metab., June 1, 2004; 89(6): 2989 - 2997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang, B. Jones Voy, S. Urs, S. Kim, M. Soltani-Bejnood, N. Quigley, Y.-R. Heo, M. Standridge, B. Andersen, M. Dhar, et al. The Human Fatty Acid Synthase Gene and De Novo Lipogenesis Are Coordinately Regulated in Human Adipose Tissue J. Nutr., May 1, 2004; 134(5): 1032 - 1038. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Hadri, M. Glorian, C. Monsempes, M.-N. Dieudonne, R. Pecquery, Y. Giudicelli, M. Andreani, I. Dugail, and B. Feve In Vitro Suppression of the Lipogenic Pathway by the Nonnucleoside Reverse Transcriptase Inhibitor Efavirenz in 3T3 and Human Preadipocytes or Adipocytes J. Biol. Chem., April 9, 2004; 279(15): 15130 - 15141. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Letexier, C. Pinteur, V. Large, V. Frering, and M. Beylot Comparison of the expression and activity of the lipogenic pathway in human and rat adipose tissue J. Lipid Res., November 1, 2003; 44(11): 2127 - 2134. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
