HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M300046-JLR200v1 44/6/1113    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vuletic, S. Articles by Albers, J. J. Search for Related Content PubMed PubMed Citation Articles by Vuletic, S. Articles by Albers, J. J. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 44, 1113-1123, June 2003
Copyright © 2003 by Lipid Research, Inc.

Widespread distribution of PLTP in human CNS

Simona Vuletic^*, Lee-Way Jin, Santica M. Marcovina^1,*, Elaine R. Peskind^**,, Thomas Möller and John J. Albers^2,*,

^* Department of Medicine, University of Washington, Seattle, WA 98195
Northwest Lipid Research Laboratories, Department of Pathology, University of Washington, Seattle, WA 98195
Department of Neurology, University of Washington, Seattle, WA 98195
^** School of Medicine, Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle, WA 98195
Mental Illness Research, Education, and Clinical Center, Veteran's Administration Puget Sound Health Care System, Seattle, WA 98108

² To whom correspondence should be addressed. e-mail: jja{at}u.washington.edu

Plasma phospholipid transfer protein (PLTP) is one of the key proteins in lipid and lipoprotein metabolism. We examined PLTP distribution in human brain using PLTP mRNA dot-blot, Northern blot, immunohistochemistry (IHC), Western blot, and phospholipid transfer activity assay analyses. PLTP mRNA of 1.8 kb was widely distributed in all the examined regions of the central nervous system at either comparable or slightly lower levels than in the other major organs, depending on the region. Cerebrospinal fluid phospholipid transfer activity represented 15% of the plasma activity, indicating active PLTP synthesis in the brain. Western blot and phosholipid transfer activity assay demonstrated secretion of active PLTP by neurons, microglia, and astrocytes in culture. IHC demonstrated PLTP presence in neurons, astrocytes, microglia, and oligodendroglia. Some neuronal groups, such as nucleus hypoglossus and CA2 neurons in hippocampus, ependymal layer, and choroid plexus were particularly strongly stained, with substantial glial and neuropil immunostaining throughout the brain.

Comparison between brain tissues from patients with Alzheimer's disease (AD) and nonAD subjects revealed a significant increase (P = 0.02) in PLTP levels in brain tissue homogenates and increased PLTP immunostaining in AD.

Abbreviations: ABC, avidin biotin complex; AD, Alzheimer's disease; CNS, central nervous system; CSF, cerebrospinal fluid; DAB, 3,3'-diaminobenzidine; FFPE, formalin-fixed, paraffin-embedded; GFAP, glial fibrillary acidic protein; HRP, horseradish peroxidase; IHC, immunohistochemistry; MAb, monoclonal antibody; MBP, myelin basic protein; PLTA, phospholipid transfer protein activity; PLTP, phospholipid transfer protein

Supplementary key words brain tissue • cerebrospinal fluid • central nervous system • immunohistochemistry

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. Settasatian, P. J. Barter, and K.-A. Rye Remodeling of apolipoprotein E-containing spherical reconstituted high density lipoproteins by phospholipid transfer protein J. Lipid Res., January 1, 2008; 49(1): 115 - 126. [Abstract] [Full Text] [PDF]

				G. M. Dallinga-Thie, A. van Tol, H. Hattori, P. C.N. Rensen, E. J.G. Sijbrands, and for the Diabetes Atorvastatin Lipid Intervention ( Plasma phospholipid transfer protein activity is decreased in type 2 diabetes during treatment with atorvastatin: a role for apolipoprotein e? Diabetes, May 1, 2006; 55(5): 1491 - 1496. [Abstract] [Full Text] [PDF]

				U. Gurok, C. Steinhoff, B. Lipkowitz, H.-H. Ropers, C. Scharff, and U. A. Nuber Gene Expression Changes in the Course of Neural Progenitor Cell Differentiation J. Neurosci., June 30, 2004; 24(26): 5982 - 6002. [Abstract] [Full Text] [PDF]

				L.-W. Jin, I. Maezawa, I. Vincent, and T. Bird Intracellular Accumulation of Amyloidogenic Fragments of Amyloid-{beta} Precursor Protein in Neurons with Niemann-Pick Type C Defects Is Associated with Endosomal Abnormalities Am. J. Pathol., March 1, 2004; 164(3): 975 - 985. [Abstract] [Full Text] [PDF]