J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1194/jlr.M300023-JLR200 on April 16, 2003

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M300023-JLR200v1
44/7/1341    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Prinsen, B. H. C. M. T.
Right arrow Articles by de Sain-van der Velden, M. G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prinsen, B. H. C. M. T.
Right arrow Articles by de Sain-van der Velden, M. G. M.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 44, 1341-1348, July 2003
Copyright © 2003 by American Society for Biochemistry and Molecular Biology

Endogenous cholesterol synthesis is associated with VLDL-2 apoB-100 production in healthy humans

Berthil H. C. M. T. Prinsen*,{dagger}, Johannes A. Romijn§, Peter H. Bisschop**, Martina M. J. de Barse{dagger}, P. Hugh R. Barrett{dagger}{dagger}, Mariëtte Ackermans**, Ruud Berger{dagger}, Ton J. Rabelink{dagger} and Monique G. M. de Sain-van der Velden1,{dagger}

* Department of Vascular Medicine and Metabolism, University Medical Center Utrecht, Utrecht, The Netherlands
{dagger} Department of Metabolic Diseases, University Medical Center Utrecht, Utrecht, The Netherlands
§ Department of Endocrinology, Leiden, University Medical Center, The Netherlands
** Department of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, The Netherlands
{dagger}{dagger} Department of Medicine, University of Western Australia and the Western Australia Institute for Medical Research, Perth, Australia

1 To whom correspondence should be addressed. e-mail: m.g.desain{at}lab.azu.nl

Subjects with high plasma cholesterol levels exhibit a high production of VLDL apolipoprotein B-100 (apoB-100), suggesting that cholesterol is a mediator for VLDL production. The objective of the study was to examine whether endogenous cholesterol synthesis, reflected by the lathosterol-cholesterol ratio (L-C ratio), affects the secretory rates of different VLDL subfractions. Ten healthy subjects were studied after overnight fasting. During a 10 h primed, constant infusion of 13C-valine (15 µmol/kg/h), enrichment was determined in apoB-100 from ultracentrifugally isolated VLDL-1 and VLDL-2 by gas chromatography mass spectrometry. The synthesis rates of VLDL-1 apoB-100 and VLDL-2 apoB-100, catabolism, and transfer were estimated by compartmental analysis. Mean VLDL-1 apoB-100 pool size was 90 ± 15 mg, and mean VLDL-2 apoB-100 pool size was 111 ± 14 mg. Absolute synthesis rate of VLDL-1 apoB-100 was 649 ± 127 mg/day and 353 ± 59 mg/day for VLDL-2 apoB-100. There was a strong association between the absolute synthesis rate of VLDL-2 apoB-100 and L-C ratio (r 2 = 0.61, P < 0.01). In contrast, no correlation was observed between L-C ratio and absolute synthesis rate of VLDL-1 apoB-100 (r 2 = 0.302, P = 0.09). In conclusion, these data provide additional support for an independent regulation of VLDL-1 apoB-100 and VLDL-2 apoB-100 production.

Endogenous cholesterol synthesis is correlated only with the VLDL-2 apoB-100 production.

Supplementary key words apolipoprotein B-100 • lathosterol • very low density lipoprotein


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
M. Adiels, S.-O. Olofsson, M.-R. Taskinen, and J. Boren
Overproduction of Very Low-Density Lipoproteins Is the Hallmark of the Dyslipidemia in the Metabolic Syndrome
Arterioscler. Thromb. Vasc. Biol., July 1, 2008; 28(7): 1225 - 1236.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
F. Basso, L. A. Freeman, C. Ko, C. Joyce, M. J. Amar, R. D. Shamburek, T. Tansey, F. Thomas, J. Wu, B. Paigen, et al.
Hepatic ABCG5/G8 overexpression reduces apoB-lipoproteins and atherosclerosis when cholesterol absorption is inhibited
J. Lipid Res., January 1, 2007; 48(1): 114 - 126.
[Abstract] [Full Text] [PDF]


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
M. C. Nierman, B. H.C.M.T. Prinsen, J. Rip, R. J. Veldman, J. A. Kuivenhoven, J. J.P. Kastelein, M. G.M. de Sain-van der Velden, and E. S.G. Stroes
Enhanced Conversion of Triglyceride-Rich Lipoproteins and Increased Low-Density Lipoprotein Removal in LPLS447X Carriers
Arterioscler. Thromb. Vasc. Biol., November 1, 2005; 25(11): 2410 - 2415.
[Abstract] [Full Text] [PDF]


Home page
Postgrad. Med. J.Home page
G D Kolovou, K K Anagnostopoulou, and D V Cokkinos
Pathophysiology of dyslipidaemia in the metabolic syndrome
Postgrad. Med. J., June 1, 2005; 81(956): 358 - 366.
[Abstract] [Full Text] [PDF]


Home page
Diabetes CareHome page
J. P.H. van Wijk, E. J.P. de Koning, M. Castro Cabezas, and T. J. Rabelink
Rosiglitazone Improves Postprandial Triglyceride and Free Fatty Acid Metabolism in Type 2 Diabetes
Diabetes Care, April 1, 2005; 28(4): 844 - 849.
[Abstract] [Full Text] [PDF]


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
L. Berglund and D. Hyson
Cholesterol Absorption and the Metabolic Syndrome: A New Look at an Old Area
Arterioscler. Thromb. Vasc. Biol., August 1, 2003; 23(8): 1314 - 1316.
[Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.