J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1194/jlr.M300059-JLR200 on June 16, 2003

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M300059-JLR200v1
44/9/1698    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Siggins, S.
Right arrow Articles by Ehnholm, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Siggins, S.
Right arrow Articles by Ehnholm, C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Lipid Research, Vol. 44, 1698-1704, September 2003
Copyright © 2003 by American Society for Biochemistry and Molecular Biology

PLTP secreted by HepG2 cells resembles the high-activity PLTP form in human plasma

Sarah Siggins, Matti Jauhiainen, Vesa M. Olkkonen, Jukka Tenhunen and Christian Ehnholm1

National Public Health Institute, Department of Molecular Medicine, Biomedicum, P.O. Box 104, FIN-00251 Helsinki, Finland

1 To whom correspondence should be addressed. e-mail: christian.ehnholm{at}ktl.fi

Plasma phospholipid transfer protein (PLTP) is an important regulator of plasma HDL levels and HDL particle distribution. PLTP is present in plasma in two forms, one with high and the other with low phospholipid transfer activity. We have used the human hepatoma cell line, HepG2, as a model to study PLTP secreted from hepatic cells. PLTP activity was secreted by the cells into serum-free culture medium as a function of time. However, modification of a previously established ELISA assay to include a denaturing sample pretreatment with the anionic detergent sodium dodecyl sulphate was required for the detection of the secreted PLTP protein. The HepG2 PLTP could be enriched by Heparin-Sepharose affinity chromatography and eluted in size-exclusion chromatography at a position corresponding to the size of 160 kDa. PLTP coeluted with apolipoprotein E (apoE) but not with apoB-100 or apoA-I. A portion of PLTP was retained by an anti-apoE immunoaffinity column together with apoE, suggesting an interaction between these two proteins. Furthermore, antibodies against apoE but not those against apoB-100 or apoA-I were capable of inhibiting PLTP activity.

These results show that the HepG2-derived PLTP resembles in several aspects the high-activity form of PLTP found in human plasma.

Abbreviations: apoA-I, apolipoprotein A-I; HRP, horseradish peroxidase; IgG, immunoglobulin G

Supplementary key words phospholipid transfer protein • apolipoprotein E • enzyme-linked immunosorbent assay • high density lipoprotein metabolism


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. Christoffersen, M. Jauhiainen, M. Moser, B. Porse, C. Ehnholm, M. Boesl, B. Dahlback, and L. B. Nielsen
Effect of Apolipoprotein M on High Density Lipoprotein Metabolism and Atherosclerosis in Low Density Lipoprotein Receptor Knock-out Mice
J. Biol. Chem., January 25, 2008; 283(4): 1839 - 1847.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
R. Liu, M. R. Hojjati, C. M. Devlin, I. H. Hansen, and X.-C. Jiang
Macrophage Phospholipid Transfer Protein Deficiency and ApoE Secretion: Impact on Mouse Plasma Cholesterol Levels and Atherosclerosis
Arterioscler. Thromb. Vasc. Biol., January 1, 2007; 27(1): 190 - 196.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
M. Lee-Rueckert, R. Vikstedt, J. Metso, C. Ehnholm, P. T. Kovanen, and M. Jauhiainen
Absence of endogenous phospholipid transfer protein impairs ABCA1-dependent efflux of cholesterol from macrophage foam cells
J. Lipid Res., August 1, 2006; 47(8): 1725 - 1732.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
M. C. Cheung and J. J. Albers
Active plasma phospholipid transfer protein is associated with apoA-I- but not apoE-containing lipoproteins
J. Lipid Res., June 1, 2006; 47(6): 1315 - 1321.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
G. M. Dallinga-Thie, A. van Tol, H. Hattori, P. C.N. Rensen, E. J.G. Sijbrands, and for the Diabetes Atorvastatin Lipid Intervention (
Plasma phospholipid transfer protein activity is decreased in type 2 diabetes during treatment with atorvastatin: a role for apolipoprotein e?
Diabetes, May 1, 2006; 55(5): 1491 - 1496.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
M. T. Janis, S. Siggins, E. Tahvanainen, R. Vikstedt, K. Silander, J. Metso, A. Aromaa, M.-R. Taskinen, V. M. Olkkonen, M. Jauhiainen, et al.
Active and low-active forms of serum phospholipid transfer protein in a normal Finnish population sample
J. Lipid Res., December 1, 2004; 45(12): 2303 - 2309.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.