J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1194/jlr.M300106-JLR200 on October 1, 2003

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M300106-JLR200v1
45/1/71    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sarkadi-Nagy, E.
Right arrow Articles by Brenna, J. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sarkadi-Nagy, E.
Right arrow Articles by Brenna, J. T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 45, 71-80, January 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology

Formula feeding potentiates docosahexaenoic and arachidonic acid biosynthesis in term and preterm baboon neonates

Eszter Sarkadi-Nagy1, Vasuki Wijendran2, Guan Yeu Diau3, Angela Chueh Chao, Andrea T. Hsieh, Anu Turpeinen4, Peter Lawrence, Peter W. Nathanielsz5 and J. Thomas Brenna6

Division of Nutritional Sciences, Savage Hall, Cornell University, Ithaca, NY 14853

6 To whom correspondence should be addressed. e-mail: jtb4{at}cornell.edu

Infant formulas supplemented with docosahexaenoic acid (DHA) and arachidonic acid (ARA) are now available in the United States; however, little is known about the factors that affect biosynthesis. Baboon neonates were assigned to one of four treatments: term, breast-fed; term, formula-fed; preterm (155 of 182 days gestation), formula-fed; and preterm, formula+DHA/ARA-fed. Standard formula had no DHA/ARA; supplemented formula had 0.61%wt DHA (0.3% of calories) and 1.21%wt ARA (0.6% of calories), and baboon breast milk contained 0.68 ± 0.22%wt DHA and 0.62 ± 0.12%wt ARA. At 14 days adjusted age, neonates received a combined oral dose of [U-13C]{alpha}-linolenic acid (LNA*) and [U-13C]linoleic acid (LA*), and tissues were analyzed 14 days after dose. Brain accretion of linolenic acid-derived DHA was ~3-fold greater for the formula groups than for the breast-fed group, and dietary DHA partially attenuated excess DHA synthesis among preterms. A similar, significant pattern was found in other organs. Brain linoleic acid-derived ARA accretion was significantly greater in the unsupplemented term group but not in the preterm groups compared with the breast-fed group.

These data show that formula potentiates the biosynthesis/accretion of DHA/ARA in term and preterm neonates compared with breast-fed neonates and that the inclusion of DHA/ARA in preterm formula partially restores DHA/ARA biosynthesis to lower, breast-fed levels. Current formula DHA concentrations are inadequate to normalize long-chain polyunsaturated fatty acids synthesis to that of breast-fed levels.

Abbreviations: ARA or 20:4n-6, arachidonic acid; CNS, central nervous system; DHA or 22:6n-3, docosahexaenoic acid; FA, fatty acid; FAME, fatty acid methyl ester; LA, linoleic acid; LA*, [U-13C]linoleic acid; LCP, long-chain polyunsaturated fatty acid; LNA, {alpha}-linolenic acid; LNA*, [U-13C]{alpha}-linolenic acid; RBC, red blood cell; RPE, retinal pigment epithelium

Supplementary key words stable isotope tracers • isotope ratio mass spectrometry • n-3 long-chain polyunsaturated fatty acids • n-6 long-chain polyunsaturated fatty acids


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
Y. H. Lin and N. Salem Jr.
Whole body distribution of deuterated linoleic and {alpha}-linolenic acids and their metabolites in the rat
J. Lipid Res., December 1, 2007; 48(12): 2709 - 2724.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
M. Igarashi, K. Ma, L. Chang, J. M. Bell, S. I. Rapoport, and J. C. DeMar Jr.
Low liver conversion rate of {alpha}-linolenic to docosahexaenoic acid in awake rats on a high-docosahexaenoate-containing diet
J. Lipid Res., August 1, 2006; 47(8): 1812 - 1822.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.