Advertisement
J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1194/jlr.M400236-JLR200 on September 1, 2004

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M400236-JLR200v1
45/11/2132    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mohr, M.
Right arrow Articles by Aigner, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mohr, M.
Right arrow Articles by Aigner, B.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 45, 2132-2137, November 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology

Hypercholesterolemia in ENU-induced mouse mutants

Manuela Mohr*, Martina Klempt*, Birgit Rathkolb*, Martin Hrabé de Angelis{dagger}, Eckhard Wolf* and Bernhard Aigner1,*

* Institute of Molecular Animal Breeding and Biotechnology, Ludwig-Maximilians-University, Munich, Germany
{dagger} Institute of Experimental Genetics, GSF Research Center for Environment and Health, Neuherberg, Germany

1 To whom correspondence should be addressed. e-mail: b.aigner{at}gen.vetmed.uni-muenchen.de

Hypercholesterolemia is caused by multiple environmental factors and genetic predispositions, and plays an important role in the development and pathogenesis of various human diseases. In this study, we aimed to establish randomly mutant mouse lines showing hypercholesterolemia for their further use in the detection of novel causative alleles. In the Munich ENU Mouse Mutagenesis Project, clinical chemistry blood analysis was performed on more than 15,000 G1 mice and 230 G3 pedigrees of chemically mutagenized mice to detect dominant and recessive mutations leading to an increased plasma total cholesterol level. Using inbred C3HeB/FeJ mice we identified more than 100 animals consistently showing hypercholesterolemia. Transmission of the altered phenotype to the subsequent generations led to the production of nine hypercholesterolemic lines. A single line showed further obvious deviations in the analysis of additional clinical chemistry blood parameters.

Thus, the lines produced will contribute to the search for alleles that selectively cause primary hypercholesterolemia.

Abbreviations: C3H, C3HeB/FeJ [inbred mouse strain]; ENU, N-ethyl-N-nitrosourea; Gn, generation number; HDL-C, HDL cholesterol; LDL-C, LDL cholesterol

Supplementary key words cholesterol • ethylnitrosourea • high density lipoproteins • low density lipoproteins • phenotype-driven screen


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Exp PhysiolHome page
B. Aigner, B. Rathkolb, M. Klaften, R. Sedlmeier, M. Klempt, S. Wagner, D. Michel, U. Mayer, T. Klopstock, M. Hrabe de Angelis, et al.
Generation of N-ethyl-N-nitrosourea-induced mouse mutants with deviations in plasma enzyme activities as novel organ-specific disease models
Exp Physiol, April 1, 2009; 94(4): 412 - 421.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
T. Juan, M. M. Veniant, J. Helmering, P. Babij, D. M. Baker, M. A. Damore, M. B. Bass, T. Gyuris, M. Chhoa, C.-M. Li, et al.
Identification of three loci affecting HDL-cholesterol levels in a screen for chemically induced recessive mutations in mice
J. Lipid Res., March 1, 2009; 50(3): 534 - 545.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
K. L. Svenson, N. Ahituv, R. S. Durgin, H. Savage, P. A. Magnani, O. Foreman, B. Paigen, and L. L. Peters
A new mouse mutant for the LDL receptor identified using ENU mutagenesis
J. Lipid Res., November 1, 2008; 49(11): 2452 - 2462.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Endocrinol. Metab.Home page
B. Aigner, B. Rathkolb, N. Herbach, M. H. de Angelis, R. Wanke, and E. Wolf
Diabetes models by screen for hyperglycemia in phenotype-driven ENU mouse mutagenesis projects
Am J Physiol Endocrinol Metab, February 1, 2008; 294(2): E232 - E240.
[Abstract] [Full Text] [PDF]


Home page
DiabetesHome page
N. Herbach, B. Rathkolb, E. Kemter, L. Pichl, M. Klaften, M. H. de Angelis, P. A. Halban, E. Wolf, B. Aigner, and R. Wanke
Dominant-Negative Effects of a Novel Mutated Ins2 Allele Causes Early-Onset Diabetes and Severe {beta}-Cell Loss in Munich Ins2C95S Mutant Mice
Diabetes, May 1, 2007; 56(5): 1268 - 1276.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Renal Physiol.Home page
B. Aigner, B. Rathkolb, N. Herbach, E. Kemter, C. Schessl, M. Klaften, M. Klempt, M. H. de Angelis, R. Wanke, and E. Wolf
Screening for increased plasma urea levels in a large-scale ENU mouse mutagenesis project reveals kidney disease models
Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1560 - F1567.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
K. Reue and L. Vergnes
Thematic review series: Systems Biology Approaches to Metabolic and Cardiovascular Disorders. Approaches to lipid metabolism gene identification and characterization in the postgenomic era
J. Lipid Res., September 1, 2006; 47(9): 1891 - 1907.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement