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Originally published In Press as doi:10.1194/jlr.M400240-JLR200 on September 1, 2004

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Journal of Lipid Research, Vol. 45, 2138-2144, November 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology

Fatty acid binding proteins stabilize leukotriene A4

: competition with arachidonic acid but not other lipoxygenase products

Jennifer S. Dickinson Zimmer*,{dagger}, Douglas F. Dyckes§, David A. Bernlohr** and Robert C. Murphy1,*,{dagger}

* Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045
{dagger} Division of Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206
§ Department of Chemistry, University of Colorado at Denver, Denver, CO 80217
** Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455

1 To whom correspondence should be addressed. e-mail: robert.murphy{at}uchsc.edu

Leukotriene A4 (LTA4) is a chemically reactive conjugated triene epoxide product derived from 5-lipoxygenase oxygenation of arachidonic acid. At physiological pH, this reactive compound has a half-life of less than 3 s at 37°C and ~40 s at 4°C. Regardless of this aqueous instability, LTA4 is an intermediate in the formation of biologically active leukotrienes, which can be formed through either intracellular or transcellular biosynthesis. Previously, epithelial fatty acid binding protein (E-FABP) present in RBL-1 cells was shown to increase the half-life of LTA4 to ~20 min at 4°C. Five FABPs (adipocyte FABP, intestinal FABP, E-FABP, heart/muscle FABP, and liver FABP) have now been examined and also found to increase the half-life of LTA4 at 4°C to ~20 min with protein present. Stabilization of LTA4 was examined when arachidonic acid was present to compete with LTA4 for the binding site on E-FABP. Arachidonate has an apparent higher affinity for E-FABP than LTA4 and was able to completely block stabilization of the latter. When E-FABP is not saturated with arachidonate, FABP can still stabilize LTA4.

Several lipoxygenase products, including 5-hydroxyeicosatetraenoic acid, 5,6-dihydroxyeicosatetraenoic acid, and leukotriene B4, were found to have no effect on the stability of LTA4 induced by E-FABP even when present at concentrations 3-fold higher than LTA4.

Abbreviations: cPLA2, cytosolic phospholipase A2; diHETE, dihydroxyeicosatetraenoic acid; EET, epoxyeicosatrienoic acid; FABP, fatty acid binding protein; FLAP, 5-lipoxygenase-activating protein; HETE, hydroxyeicosatetraenoic acid; HpETE, hydroperoxyeicosatetraenoic acid; LTA4, leukotriene A4; LTB4, leukotriene B4; TEA, triethylamine; 5-LO, 5-lipoxygenase

Supplementary key words half-life • transcellular biosynthesis • leukotriene biosynthesis


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