HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M400185-JLR200v1 45/12/2235    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Conde-Knape, K. Articles by Shachter, N. S. Search for Related Content PubMed PubMed Citation Articles by Conde-Knape, K. Articles by Shachter, N. S. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 45, 2235-2244, December 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology

Overexpression of apoC-III produces lesser hypertriglyceridemia in apoB-48-only gene-targeted mice than in apoB-100-only mice

Karin Conde-Knape^1,*, Kenta Okada^2,*, Rajasekhar Ramakrishnan and Neil S. Shachter^3,*

^* Departments of Medicine, Columbia University, New York, NY 10032
Pediatrics, Columbia University, New York, NY 10032

³ To whom correspondence should be addressed. e-mail: nss5{at}columbia.edu

The adaptive value of apolipoprotein B-48 (apoB-48), the truncated form of apoB produced by the intestine, in lipid metabolism remains unclear. We crossed human apoC-III transgenic mice with mice expressing either apoB-48 only (apoB^48/48) or apoB-100 only (apoB^100/100). Cholesterol levels were higher in apoB^48/48 mice than in apoB^100/100 mice but triglyceride levels were similar. Lipid levels were increased by the apoC-III transgene. However, triglyceride levels were significantly higher in apoB^100/100C-III than in apoB^48/48C-III mice (895 ± 395 mg/dl vs. 690 ± 252 mg/dl; P < 0.01), whereas cholesterol levels were higher in the apoB^48/48C-III mice than in apoB^100/100C-III (144 ± 35 mg/dl vs. 94 ± 30 mg/dl; P < 0.00001). Triglyceride clearance from VLDL was impaired to a greater extent in apoB^100/100C-III vs. apoB^100/100 mice than in apoB^48/48C-III vs. apoB^48/48 mice. Triglyceride secretion rates were no different in apoC-III transgenic mice than in their nontransgenic littermates. ApoB-48 triglyceride-rich lipoproteins were more resistant to the triglyceride-increasing effects of apoC-III but appeared more sensitive to the remnant clearance inhibition.

Our findings support a coordinated role for apoB-48 in facilitating the delivery of dietary triglycerides to the periphery. Consistent with such a mechanism, glucose levels were significantly higher in apoB^48/48 mice vs. apoB^100/100 mice, perhaps on the basis of metabolic competition.

Supplementary key words apolipoproteins C • apolipoproteins B • hyperglycemia • transgenic • postprandial period

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?