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Journal of Lipid Research, Vol. 45, 281-286, February 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology

Characterization of the ceramide moieties of sphingoglycolipids from mouse brain by ESI-MS/MS

Benoit Colsch^*,, Carlos Afonso, Iuliana Popa, Jacques Portoukalian, Françoise Fournier, Jean-Claude Tabet and Nicole Baumann^1,*

^* Institut National de la Santé et de la Recherche Médicale U495, Laboratoire de Neurochimie, Hopital de la Salpetriere, 75651 Paris cedex 13, France
Centre National de la Recherche Scientifique Unité Mixte de Recherche 7613, Structure et Fonction de Molecules Bioactives, Universite Pierre et Marie Curie, 75252 Paris cedex 05, France
Institut National de la Santé et de la Recherche Médicale U346, Laboratoire de Dermatologie, Hopital Edouard Herriot, 69003 Lyon, France

¹ To whom correspondence should be addressed. e-mail: baumann{at}ccr.jussieu.fr

Sphingoglycolipids (SGLs) are cell membrane constituents. As the ceramide structure influences the biological properties of the SGL, we characterized by electrospray ionization tandem mass spectrometry the molecular species of ceramides present in SGL of mouse brain. We report here for the first time the presence in mammalian brain of sphingadienine (d18:2). Sphingenine (d18:1) is present in all SGL species, in contrast to eicosasphingenine (d20:1), which is a constituent of only gangliosides. Sphingadienine is present in galactosylceramide and sulfatides. Free ceramides contain the three types of bases.

Thus, there could be two separate pools of free ceramides (d18:1, d18:2 and d20:1, d18:1) as precursors of complex SGL.

Supplementary key words ion trap • triple quadrupole • precursor ion scan • electrospray ionization tandem mass spectrometry

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