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Journal of Lipid Research, Vol. 45, 295-300, February 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology

Presence of protein-bound unconjugated bile acids in the cytoplasmic fraction of rat brain

Nariyasu Mano^1,*, Takaaki Goto^*, Masashi Uchida^*, Koji Nishimura^*, Masayuki Ando, Norihiro Kobayashi and Junichi Goto^*,**

^* Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-8578, Japan
Department of Analytical Chemistry, Niigata College of Pharmacy, 5-13-2 Kamishinei-cho, Niigata 950-2081, Japan
Kobe Pharmaceutical University, 4-9-1 Motoyama-Kitamachi, Higashinada-ku, Kobe 658-8558, Japan
^** Department of Pharmaceutical Sciences, Tohoku University Hospital, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan

¹ To whom correspondence should be addressed. e-mail: n-mano{at}mail.pharm.tohoku.ac.jp

Using liquid chromatography/electrospray ionization mass spectrometry, we have found three unconjugated bile acids [cholic acid (CA), chenodeoxycholic acid (CDCA), and deoxycholic acid (DCA)] in the rat brain cytoplasmic fraction. CDCA was detected only upon extraction with high concentrations of guanidine, indicating that it is bound noncovalently to protein in the brain. The most abundant of the three, it was present at a concentration of 1.6 nmol/g wet weight (15 mg of protein) of brain, corresponding to almost 30 times its serum concentration. CA and DCA were present at 1/30th the concentration of CDCA. Bile acids conjugated with amino acids, sulfuric acid, and glucuronic acid were not detected.

These data clearly demonstrate that unconjugated CDCA and, to a lesser extent, CA and DCA, exists in the rat brain.

Abbreviations: CA, cholic acid; CDCA, chenodeoxycholic acid; DCA, deoxycholic acid; ESI, electrospray ionization; IS, internal standard; LC/MS, liquid chromatography/mass spectrometry; SIM, selected ion monitoring

Supplementary key words chenodeoxycholic acid • cholic acid • deoxycholic acid • liquid chromatography/mass spectrometry

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