J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1194/jlr.M300440-JLR200 on December 16, 2003

Papers In Press, published online ahead of print March 1, 2004
J. Lipid Res., doi:10.1194/jlr.M300440-JLR200
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M300440-JLR200v1
45/3/427    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wüstner, D.
Right arrow Articles by Maxfield, F. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wüstner, D.
Right arrow Articles by Maxfield, F. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Lipid Research, Vol. 45, 427-437, March 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology

Different transport routes for high density lipoprotein and its associated free sterol in polarized hepatic cells

Daniel Wüstner1, Mousumi Mondal, Amy Huang and Frederick R. Maxfield2

Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021

2 To whom correspondence should be addressed. e-mail: frmaxfie{at}med.cornell.edu

We analyzed the intracellular transport of HDL and its associated free sterol in polarized human hepatoma HepG2 cells. Using pulse-chase protocols, we demonstrated that HDL labeled with Alexa 488 at the apolipoprotein (Alexa 488-HDL) was internalized by a scavenger receptor class B type I (SR-BI)-dependent process at the basolateral membrane and became enriched in a subapical/apical recycling compartment. Most Alexa 488-HDL was rapidly recycled to the basolateral cell surface and released from cells. Within 30 min of chase at 37°C, ~3% of the initial cell-associated Alexa 488-HDL accumulated in the biliary canaliculus (BC) formed at the apical pole of polarized HepG2 cells. Even less Alexa 488-HDL was transported to late endosomes or lysosomes. The fluorescent cholesterol analog dehydroergosterol (DHE) incorporated into Alexa 488-HDL was delivered to the BC within a few minutes, independent of the labeled apolipoprotein. This transport did not require metabolic energy and could be blocked by antibodies against SR-BI. The fraction of cell-associated DHE transported to the BC was comparable when cells were incubated with either Alexa 488-HDL containing DHE or with DHE bound to methyl-ß-cyclodextrin.

We conclude that rapid, nonvesicular transport of sterol to the BC and efficient recycling of HDL particles underlies the selective sorting of sterol from HDLs in hepatocytes.

Abbreviations: Alexa 488-HDL, Alexa 488-labeled high density lipoprotein; apoA-I, apolipoprotein A-I; BC, biliary canaliculus; DHE, dehydroergosterol; DHE/Alexa 488-HDL, Alexa 488-labeled high density lipoprotein containing dehydroergosterol; DHE/MßCD, DHE loaded on methyl-ß-cyclodextrin; DiI-C18 dioctadecanoyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate; ERC, endocytic recycling compartment; MßCD, methyl-ß-cyclodextrin; PC, phosphatidylcholine; PFA, paraformaldehyde; ROI, region of interest; SAC/ARC, subapical compartment/apical recycling compartment; SR-BI, scavenger receptor class B type I; Tf, transferrin

Supplementary key words hepatocytes • dehydroergosterol • bile • fluorescence microscopy


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
T. A. Pagler, A. Neuhofer, H. Laggner, W. Strobl, and H. Stangl
Cholesterol efflux via HDL resecretion occurs when cholesterol transport out of the lysosome is impaired
J. Lipid Res., October 1, 2007; 48(10): 2141 - 2150.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
Y. Zhang, A. M. Ahmed, N. McFarlane, C. Capone, D. R. Boreham, R. Truant, S. A. Igdoura, and B. L. Trigatti
Regulation of SR-BI-mediated selective lipid uptake in Chinese hamster ovary-derived cells by protein kinase signaling pathways
J. Lipid Res., February 1, 2007; 48(2): 405 - 416.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. J. Harder, A. Meng, P. Rippstein, H. M. McBride, and R. McPherson
SR-BI Undergoes Cholesterol-stimulated Transcytosis to the Bile Canaliculus in Polarized WIF-B Cells
J. Biol. Chem., January 12, 2007; 282(2): 1445 - 1455.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. Wustner
Plasma Membrane Sterol Distribution Resembles the Surface Topography of Living Cells
Mol. Biol. Cell, January 1, 2007; 18(1): 211 - 228.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
B. Sun, E. R. M. Eckhardt, S. Shetty, D. R. van der Westhuyzen, and N. R. Webb
Quantitative analysis of SR-BI-dependent HDL retroendocytosis in hepatocytes and fibroblasts
J. Lipid Res., August 1, 2006; 47(8): 1700 - 1713.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
N. A. Braun, P. J. Mohler, K. H. Weisgraber, A. H. Hasty, M. F. Linton, P. G. Yancey, Y. R. Su, S. Fazio, and L. L. Swift
Intracellular trafficking of recycling apolipoprotein E in Chinese hamster ovary cells
J. Lipid Res., June 1, 2006; 47(6): 1176 - 1186.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. A. Pagler, S. Rhode, A. Neuhofer, H. Laggner, W. Strobl, C. Hinterndorfer, I. Volf, M. Pavelka, E. R. M. Eckhardt, D. R. van der Westhuyzen, et al.
SR-BI-mediated High Density Lipoprotein (HDL) Endocytosis Leads to HDL Resecretion Facilitating Cholesterol Efflux
J. Biol. Chem., April 21, 2006; 281(16): 11193 - 11204.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
C. J. Harder, G. Vassiliou, H. M. McBride, and R. McPherson
Hepatic SR-BI-mediated cholesteryl ester selective uptake occurs with unaltered efficiency in the absence of cellular energy
J. Lipid Res., March 1, 2006; 47(3): 492 - 503.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. R. M. Eckhardt, L. Cai, S. Shetty, Z. Zhao, A. Szanto, N. R. Webb, and D. R. Van der Westhuyzen
High Density Lipoprotein Endocytosis by Scavenger Receptor SR-BII Is Clathrin-dependent and Requires a Carboxyl-terminal Dileucine Motif
J. Biol. Chem., February 17, 2006; 281(7): 4348 - 4353.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Wustner
Mathematical Analysis of Hepatic High Density Lipoprotein Transport Based on Quantitative Imaging Data
J. Biol. Chem., February 25, 2005; 280(8): 6766 - 6779.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Cai, M. C. de Beer, F. C. de Beer, and D. R. van der Westhuyzen
Serum Amyloid A Is a Ligand for Scavenger Receptor Class B Type I and Inhibits High Density Lipoprotein Binding and Selective Lipid Uptake
J. Biol. Chem., January 28, 2005; 280(4): 2954 - 2961.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. C.D. van IJzendoorn, J. M. van der Wouden, G. Liebisch, G. Schmitz, and D. Hoekstra
Polarized Membrane Traffic and Cell Polarity Development Is Dependent on Dihydroceramide Synthase-Regulated Sphinganine Turnover
Mol. Biol. Cell, September 1, 2004; 15(9): 4115 - 4124.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.