J. Lipid Res.
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Originally published In Press as doi:10.1194/jlr.M300242-JLR200 on December 16, 2003

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Journal of Lipid Research, Vol. 45, 456-465, March 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology

Lipid transfer particle mediates the delivery of diacylglycerol from lipophorin to fat body in larval Manduca sexta

Lilian E. Canavoso1, Hwa Kyung Yun2, Zeina E. Jouni3 and Michael A. Wells

Department of Biochemistry and Molecular Biophysics and Center for Insect Science, Biological Sciences West, University of Arizona, Tucson, AZ 85721-0088

3 To whom correspondence should be addressed. e-mail: eljouniz{at}email.arizona.edu

This work analyzed the process of lipid storage in fat body of larval Manduca sexta, focusing on the role of lipid transfer particle (LTP). Incubation of fat bodies with [3H]diacylglycerol-labeled lipophorin resulted in a significant accumulation of diacylglycerol (DAG) and triacylglycerol (TAG) in the tissue. Transfer of DAG to fat body and its storage as TAG was significantly inhibited (60%) by preincubating the tissue with anti-LTP antibody. Lipid transfer was restored to control values by adding LTP to fat body. Incubation of fat body with dual-labeled DAG lipophorin or its treatment with ammonium chloride showed that neither a membrane-bound lipoprotein lipase nor lipophorin endocytosis is a relevant pathway to transfer or to storage lipids into fat body, respectively. Treatment of fat body with suramin caused a 50% inhibition in [3H]DAG transfer from lipophorin. Treatment of [3H]DAG-labeled fat body with lipase significantly reduced the amount of [3H]DAG associated with the tissue, suggesting that the lipid is still on the external surface of the membrane.

Whether this lipid represents irreversibly adsorbed lipophorin or a DAG lipase-sensitive pool is unknown. Nevertheless, these results indicate that the main pathway for DAG transfer from lipophorin to fat body is via LTP and receptor-mediated processes.

Supplementary key words lipoprotein lipase • developmental stages • triacylglycerol • endocytosis


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Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.