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J. Lipid Res.
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Originally published In Press as doi:10.1194/jlr.D300035-JLR200 on January 16, 2004

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Journal of Lipid Research, Vol. 45, 773-775, April 2004
Copyright © 2004 by American Society for Biochemistry and Molecular Biology

Methods

Improved method for making nondenaturing composite gradient gels for the electrophoretic separation of lipoproteins

David L. Rainwater1, Perry H. Moore, Jr. and Israel O. Gamboa

Department of Genetics, Southwest Foundation for Biomedical Research, PO Box 760549, San Antonio, TX 78245-0549

1 To whom correspondence should be addressed. e-mail: david{at}darwin.sfbr.org

Nondenaturing gradient gel electrophoresis continues to be used widely for resolution and characterization of lipoprotein subclasses. Methods for making such gels in the laboratory have been published, but occasionally samples do not display uniform mobilities for all lanes in a laboratory-made gel.

To help overcome this limitation, we recommend a modification

Supplementary key words gradient gel electrophoresis • lipoprotein separation • human • baboon


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