HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: C400004-JLR200v1 46/1/21    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lembcke, J. Articles by Thiery, J. Search for Related Content PubMed PubMed Citation Articles by Lembcke, J. Articles by Thiery, J. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 46, 21-26, January 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

Rapid Communication

Rapid quantification of free and esterified phytosterols in human serum using APPI-LC-MS/MS

Jan Lembcke¹, Uta Ceglarek¹, Georg Martin Fiedler, Sven Baumann, Alexander Leichtle and Joachim Thiery²

Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University Hospital, Leipzig, Germany

² To whom correspondence should be addressed. e-mail: thiery{at}medizin.uni-leipzig.de

ABSTRACT

A novel analytical platform based on liquid chromatography and tandem mass spectrometry using atmospheric pressure photoionization was applied for the simultaneous quantification of free and esterified ß-sitosterol, campesterol, brassicasterol, and stigmasterol. The total time for sample pretreatment and analysis could be reduced from 3 h [gas chromatography-mass spectrometry (GC-MS)] to 15 min. The detection limits of the different phytosterols ranged between 0.25 and 0.68 µg/l. Linear ranges were between 1 and 1,000 µg/l. The within-run and between-run variabilities ranged between 1.4% and 9.9%. The analytical sensitivity was at least 150-fold higher compared with GC-MS.

Our new method allows a rapid and simultaneous determination of free and esterified phytosterols in serum.

Abbreviations: APPI, atmospheric pressure photoionization; LC-MS/MS, liquid chromatography-tandem mass spectrometry; LPDS, lipoprotein-deficient serum

Supplementary key words free phytosterols • phytosterol esters • liquid chromatography • plant sterols

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. H. Chase, B. L. Lyons, R. T. Bronson, O. Foreman, L. R. Donahue, L. M. Burzenski, B. Gott, P. Lane, B. Harris, U. Ceglarek, et al. The mouse mutation "thrombocytopenia and cardiomyopathy" (trac) disrupts Abcg5: a spontaneous single gene model for human hereditary phytosterolemia/sitosterolemia Blood, February 11, 2010; 115(6): 1267 - 1276. [Abstract] [Full Text] [PDF]

				A. Honda, K. Yamashita, H. Miyazaki, M. Shirai, T. Ikegami, G. Xu, M. Numazawa, T. Hara, and Y. Matsuzaki Highly sensitive analysis of sterol profiles in human serum by LC-ESI-MS/MS J. Lipid Res., September 1, 2008; 49(9): 2063 - 2073. [Abstract] [Full Text] [PDF]

				S Kidambi and S B Patel Sitosterolaemia: pathophysiology, clinical presentation and laboratory diagnosis J. Clin. Pathol., May 1, 2008; 61(5): 588 - 594. [Abstract] [Full Text] [PDF]

				K. Karu, M. Hornshaw, G. Woffendin, K. Bodin, M. Hamberg, G. Alvelius, J. Sjovall, J. Turton, Y. Wang, and W. J. Griffiths Liquid chromatography-mass spectrometry utilizing multi-stage fragmentation for the identification of oxysterols J. Lipid Res., April 1, 2007; 48(4): 976 - 987. [Abstract] [Full Text] [PDF]

				E. Sehayek, Y. Y. Fung, H. J. Yu, J. Lembcke, U. Ceglarek, D. Teupser, J. Thiery, D. Lutjohann, K. von Bergmann, and J. L. Breslow A complex plasma plant sterol locus on mouse chromosome 14 has at least two genes regulating intestinal sterol absorption J. Lipid Res., October 1, 2006; 47(10): 2291 - 2296. [Abstract] [Full Text] [PDF]