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Journal of Lipid Research, Vol. 46, 2265-2277, October 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

Relation between insulin resistance and fast-migrating LDL subfraction as characterized by capillary isotachophoresis

Bo Zhang^1,*, Takuya Kaneshi, Takao Ohta and Keijiro Saku^*

^* Department of Cardiology, Fukuoka University School of Medicine, Fukuoka, Japan
Department of Pediatrics, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan

Published, JLR Papers in Press, August 1, 2005. DOI 10.1194/jlr.M500192-JLR200

¹ To whom correspondence should be addressed. e-mail: bozhang{at}fukuoka-u.ac.jp

in re-

The proportion of the electronegative low density lipoprotein [LDL(–)] subfraction, which is atherogenic, is increased in type 2 diabetes but is not reduced by glycemic control. Therefore, we evaluated the ability of a new technique, capillary isotachophoresis (cITP), to quantify charge-based LDL subfractions and examined the relation between insulin resistance and the cITP fast-migrating (f) LDL levels. Seventy-five 10-year-old boys were included. The two cITP LDL subfractions, fLDL and major LDL subfractions, were proportional to the LDL protein content within the range of 0.1–0.8 mg/ml LDL protein. Levels of cITP fLDL were positively correlated with triglyceride (TG) levels and negatively correlated with LDL size. Insulin resistance as assessed by the homeostasis model assessment (HOMA-IR) was positively correlated (P < 0.01) with cITP fLDL levels (r = 0.41). The relation between HOMA-IR and cITP fLDL levels depended on TG levels but was independent of body mass index and LDL size.

cITP lipoprotein analysis is an accurate and sensitive method for quantifying charge-based LDL subfractions in human plasma, and insulin resistance is related to cITP fLDL independent of LDL size.

Supplementary key words children • plasma lipoprotein subfraction • low density lipoprotein size • electronegative low density lipoprotein

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