J. Lipid Res. Please sign the JLR Guestbook
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1194/jlr.M500185-JLR200 on August 1, 2005

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M500185-JLR200v1
46/10/2295    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bamba, T.
Right arrow Articles by Kobayashi, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bamba, T.
Right arrow Articles by Kobayashi, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 46, 2295-2298, October 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

Methods

Separation of polyprenol and dolichol by monolithic silica capillary column chromatography

Takeshi Bamba*,{dagger}, Eiiciro Fukusaki1,*, Hiroshi Minakuchi§, Yoshihisa Nakazawa{dagger} and Akio Kobayashi*

* Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
{dagger} Hitachi Zosen Corporation, 2-2-11 Funamachi, Taisyou-ku, Osaka 551-0022, Japan
§ Kyoto Monotech, 13 Shimotsubayashi Shibanomiya-cho, Nishikyo-ku, Kyoto, Kyoto 615- 8035, Japan

Published, JLR Papers in Press, August 1, 2005. DOI 10.1194/jlr.M500185-JLR200

1 To whom correspondence should be addressed. e-mail: fukusaki{at}bio.eng.osaka-u.ac.jp

We attempted an analysis of naturally occurring polyprenol and dolichol using a monolithic silica capillary column in HPLC. First, the separation of the polyprenol mixture alone was performed using a 250 x 0.2 mm inner diameter (ID) octadecylsilyl (ODS)-monolithic silica capillary column. The resolution of the separation between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) exceeded by ≥2-fold the level recorded when using a conventional ODS-silica particle-packed column (250 x 4.6 mm ID) under the same elution conditions. Next, the mixture of the prenol type (polyprenol) and dolichol type (dihydropolyprenol) was subjected to this capillary HPLC system, and the separation of each homolog was successfully achieved. During the analysis of polyprenol fraction derived from Eucommia ulmoides leaves, dolichols were found as a single peak, including all-trans-polyprenol and cis-polyprenol previously identified.

This sensitive high-resolution system is very useful for the analysis of compounds that are structurally close to polyprenols and dolichols and that have a low content.

Abbreviations: ODS, octadecylsilyl; SFC, supercritical fluid chromatography

Supplementary key words high-performance liquid chromatography • profiling • metabolomics • metabolome


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.