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Originally published In Press as doi:10.1194/jlr.M500168-JLR200 on August 16, 2005

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Journal of Lipid Research, Vol. 46, 2388-2397, November 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters

Chi-Liang Eric Yen1,*,{dagger}, Charles H. Brown, IV*, Mara Monetti*,{dagger} and Robert V. Farese, Jr.*,{dagger},§

* Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158
{dagger} Cardiovascular Research Institute, University of California, San Francisco, CA 94143
§ Department of Medicine, University of California, San Francisco, CA 94143

Published, JLR Papers in Press, August 16, 2005. DOI 10.1194/jlr.M500168-JLR200

1 To whom correspondence should be addressed. e-mail: eyen{at}gladstone.ucsf.edu

Acyl-CoA-dependent O-acyltransferases catalyze reactions in which fatty acyl-CoAs are joined to acyl acceptors containing free hydroxyl groups to produce neutral lipids. In this report, we characterize a human multifunctional O-acyltransferase (designated MFAT) that belongs to the acyl-CoA:diacylglycerol acyltransferase 2/acyl-CoA:monoacylglycerol acyltransferase (MGAT) gene family and is highly expressed in the skin. Membranes of insect cells and homogenates of mammalian cells overexpressing MFAT exhibited significantly increased MGAT, acyl-CoA:fatty acyl alcohol acyltransferase (wax synthase), and acyl-CoA:retinol acyltransferase (ARAT) activities, which catalyze the synthesis of diacylglycerols, wax monoesters, and retinyl esters, respectively. Furthermore, when provided with the appropriate substrates, intact mammalian cells overexpressing MFAT accumulated more waxes and retinyl esters than control cells.

We conclude that MFAT is a multifunctional acyltransferase that likely plays an important role in lipid metabolism in human skin.

Abbreviations: ARAT, acyl-coenzyme A:retinol acyltransferase; DGAT, acyl-coenzyme A:diacylglycerol acyltransferase; MFAT, multifunctional acyltransferase; MGAT, acyl-coenzyme A:monoacylglycerol acyltransferase; NCBI, National Center for Biotechnology Information

Supplementary key words neutral lipids • diacylglycerol • fatty alcohol • esterification


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