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Journal of Lipid Research, Vol. 46, 2649-2656, December 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

Lipid mixtures prepared with well-defined synthetic ceramides closely mimic the unique stratum corneum lipid phase behavior

Miranda W. de Jager^*, Gert S. Gooris^*, Maria Ponec and Joke A. Bouwstra^1,*

^* Department of Drug Delivery Technology, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands

Published, JLR Papers in Press, September 8, 2005. DOI 10.1194/jlr.M500221-JLR200

¹ To whom correspondence should be addressed. e-mail: bouwstra{at}chem.leidenuniv.nl

Lipid lamellae present in the outermost layer of the skin, the stratum corneum, form the main barrier for the diffusion of molecules through the skin. The presence of a unique 13 nm lamellar phase and its high crystallinity are characteristic for the stratum corneum lipid phase behavior. In the present study, small-angle and wide-angle X-ray diffraction were used to examine the organization in lipid mixtures prepared with a unique set of well-defined synthetic ceramides, varying from each other in head group architecture and acyl chain length. The results show that equimolar mixtures of cholesterol, free fatty acids, and synthetic ceramides (resembling the composition of pig ceramides) closely resemble the lamellar and lateral stratum corneum lipid organization, both at room and higher temperatures. Exclusion of several ceramide classes from the mixture does not affect the lipid organization. However, complete substitution of ceramide 1 (acylceramide with a sphingosine base) with ceramide 9 (acylceramide with a phytosphingosine base) reduces the formation of the long periodicity lamellar phase. This indicates that the head group architecture of acylceramides affects the lipid organization.

In conclusion, lipid mixtures prepared with well-defined synthetic ceramides offer an attractive tool with which to unravel the importance of the molecular structure of individual ceramides for proper lipid organization.

Supplementary key words barrier • X-ray diffraction • lipid organization

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