J. Lipid Res. Please sign the JLR Guestbook
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1194/jlr.M400212-JLR200 on February 1, 2005

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M400212-JLR200v1
46/5/896    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jahangiri, A.
Right arrow Articles by Rye, K-A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jahangiri, A.
Right arrow Articles by Rye, K-A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 46, 896-903, May 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

Evidence that endothelial lipase remodels high density lipoproteins without mediating the dissociation of apolipoprotein A-I

A. Jahangiri1,*, D. J. Rader{dagger}, D. Marchadier{dagger}, L. K. Curtiss§, D. J. Bonnet§ and K-A. Rye*,**,{dagger}{dagger}

* Lipid Research Group, Heart Research Institute, Camperdown, Sydney, New South Wales 2050, Australia
{dagger} Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
§ Department of Immunology, Scripps Research Institute, La Jolla, CA 92037
** Department of Medicine, University of Sydney, Sydney, New South Wales 2006, Australia
{dagger}{dagger} Department of Medicine, University of Melbourne, Melbourne, Victoria 3010, Australia

1 To whom correspondence should be addressed. e-mail: a.jahangiri{at}hri.org.au

Endothelial lipase (EL) is a triglyceride lipase gene family member that has high phospholipase and low triglyceride lipase activity. The aim of this study was to determine whether the phospholipase activity of EL is sufficient to remodel HDLs into small particles and mediate the dissociation of apolipoprotein A-I (apoA-I). Spherical, reconstituted HDLs (rHDLs) containing apoA-I only [(A-I)rHDLs], apoA-II only [(A-II)rHDLs], or both apoA-I and apoA-II [(A-I/A-II) rHDLs] were prepared. The rHDLs, which contained only cholesteryl esters in their core and POPC on the surface, were incubated with EL. As the rHDLs did not contain triacylglycerol, only the POPC was hydrolyzed. Hydrolysis was greater in the (A-I/A-II)rHDLs than in the (A-I)rHDLs. The (A-II)rHDL phospholipids were not hydrolyzed by EL. EL remodeled the (A-I)rHDLs and (A-I/A-II)rHDLs, but not the (A-II)rHDLs, into smaller particles. The reduction in particle size was related to the amount of phospholipid hydrolysis, with the diameter of the (A-I/A-II)rHDLs decreasing more than that of the (A-I)rHDLs. These changes did not affect the conformation of apoA-I, and neither apoA-I nor apoA-II dissociated from the rHDLs. Comparable results were obtained when human plasma HDLs were incubated with EL.

These results establish that the phospholipase activity of EL remodels plasma HDLs and rHDLs into smaller particles without mediating the dissociation of apolipoproteins.

Abbreviations: apoA-I, apolipoprotein A-I; CE, cholesteryl ester; CETP, cholesteryl ester transfer protein; EL, endothelial lipase; RAM-Fc, rabbit anti-mouse Fc; rHDL, reconstituted high density lipoprotein; TG, triacylglycerol; UC, unesterified cholesterol

Supplementary key words phospholipids • hydrolysis • lipoprotein metabolism


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
N. Settasatian, P. J. Barter, and K.-A. Rye
Remodeling of apolipoprotein E-containing spherical reconstituted high density lipoproteins by phospholipid transfer protein
J. Lipid Res., January 1, 2008; 49(1): 115 - 126.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. A. G. D. Silva, L. A. Schneeweis, S. C. Krishnan, X. Zhang, P. H. Axelsen, and W. S. Davidson
The Structure of Apolipoprotein A-II in Discoidal High Density Lipoproteins
J. Biol. Chem., March 30, 2007; 282(13): 9713 - 9721.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
L. K. Curtiss, D. T. Valenta, N. J. Hime, and K.-A. Rye
What Is So Special About Apolipoprotein AI in Reverse Cholesterol Transport?
Arterioscler. Thromb. Vasc. Biol., January 1, 2006; 26(1): 12 - 19.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.