| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Journal of Lipid Research, Vol. 46, 1331-1338, June 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology
Institute for Arteriosclerosis Research, University of Münster, Münster, Germany
Published, JLR Papers in Press, March 1, 2005. DOI 10.1194/jlr.M400323-JLR200
1 To whom correspondence should be addressed. e-mail: robenek{at}uni-muenster.de
The PAT family proteins, named after perilipin, adipophilin, and the tail-interacting protein of 47 kDa (TIP47), are implicated in intracellular lipid metabolism. They associate with lipid droplets, but how is completely unclear. From immunofluorescence studies, they are reported to be restricted to the outer membrane monolayer enveloping the lipid droplet and not to enter the core. Recently, we found another kind of lipid droplet-associated protein, caveolin-1, inside lipid droplets. Using freeze-fracture immunocytochemistry and electron microscopy, we now describe the distributions of perilipin and caveolin-1 and of adipophilin and TIP47 in lipid droplets of adipocytes and macrophages. All of these lipid droplet-associated proteins pervade the lipid droplet core and hence are not restricted to the droplet surface. Moreover, lipid droplets are surprisingly heterogeneous with respect to their complements and their distribution of lipid droplet-associated proteins. Whereas caveolin-1 is synthesized in the endoplasmic reticulum and is transferred to the lipid droplet core by inundating lipids during droplet budding, the PAT proteins, which are synthesized on free ribosomes in the cytoplasm, evidently target to the lipid droplet after it has formed.
How the polar lipid droplet-associated proteins are accommodated among the essentially hydrophobic neutral lipids of the lipid droplet core remains to be determined.
Supplementary key words perilipin • adipophilin • tail-interacting protein of 47 kDa • freeze-fracture labeling
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. M. Goodman The Gregarious Lipid Droplet J. Biol. Chem., October 17, 2008; 283(42): 28005 - 28009. [Full Text] [PDF]
|
|
|
|
 |
|
 G. Murphy Jr., R. L. Rouse, W. W. Polk, W. G. Henk, S. A. Barker, M. J. Boudreaux, Z. E. Floyd, and A. L. Penn Combustion-Derived Hydrocarbons Localize to Lipid Droplets in Respiratory Cells Am. J. Respir. Cell Mol. Biol., May 1, 2008; 38(5): 532 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Muller, S. Wied, N. Walz, and C. Jung Translocation of Glycosylphosphatidylinositol-Anchored Proteins from Plasma Membrane Microdomains to Lipid Droplets in Rat Adipocytes Is Induced by Palmitate, H2O2, and the Sulfonylurea Drug Glimepiride Mol. Pharmacol., May 1, 2008; 73(5): 1513 - 1529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. H.-J. Chang and L. Chan Regulation of Triglyceride Metabolism. III. Emerging role of lipid droplet protein ADFP in health and disease Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1465 - G1468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-C. Wan, R. C. N. Melo, Z. Jin, A. M. Dvorak, and P. F. Weller Roles and origins of leukocyte lipid bodies: proteomic and ultrastructural studies FASEB J, January 1, 2007; 21(1): 167 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Robenek, O. Hofnagel, I. Buers, M. J. Robenek, D. Troyer, and N. J. Severs Adipophilin-enriched domains in the ER membrane are sites of lipid droplet biogenesis J. Cell Sci., October 15, 2006; 119(20): 4215 - 4224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hanisch, M. Waltermann, H. Robenek, and A. Steinbuchel Eukaryotic lipid body proteins in oleogenous actinomycetes and their targeting to intracellular triacylglycerol inclusions: impact on models of lipid body biogenesis. Appl. Envir. Microbiol., October 1, 2006; 72(10): 6743 - 6750. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Robenek, O. Hofnagel, I. Buers, S. Lorkowski, M. Schnoor, M. J. Robenek, H. Heid, D. Troyer, and N. J. Severs Butyrophilin controls milk fat globule secretion PNAS, July 5, 2006; 103(27): 10385 - 10390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamaguchi, S. Matsushita, K. Motojima, F. Hirose, and T. Osumi MLDP, a Novel PAT Family Protein Localized to Lipid Droplets and Enriched in the Heart, Is Regulated by Peroxisome Proliferator-activated Receptor {alpha} J. Biol. Chem., May 19, 2006; 281(20): 14232 - 14240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Kleinbongard, R. Schulz, T. Rassaf, T. Lauer, A. Dejam, T. Jax, I. Kumara, P. Gharini, S. Kabanova, B. Ozuyaman, et al. Red blood cells express a functional endothelial nitric oxide synthase Blood, April 1, 2006; 107(7): 2943 - 2951. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shimizu, Mst. H. Akter, Y. Emi, R. Sato, T. Yamaguchi, F. Hirose, and T. Osumi Peroxisome Proliferator-Activated Receptor Subtypes Differentially Cooperate with Other Transcription Factors in Selective Transactivation of the Perilipin/PEX11{alpha} Gene Pair. J. Biochem., March 1, 2006; 139(3): 563 - 573. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. H.-J. Chang, L. Li, A. Paul, S. Taniguchi, V. Nannegari, W. C. Heird, and L. Chan Protection against Fatty Liver but Normal Adipogenesis in Mice Lacking Adipose Differentiation-Related Protein Mol. Cell. Biol., February 1, 2006; 26(3): 1063 - 1076. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
