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Journal of Lipid Research, Vol. 46, 1388-1395, July 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy

David Touboul^*, Alain Brunelle^1,*, Frédéric Halgand^*, Sabine De La Porte and Olivier Laprévote^*

^* Laboratoire de Spectrométrie de Masse, Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2301, F91198 Gif sur Yvette Cedex, France
Laboratoire de Neurobiologie Cellulaire et Moléculaire, Institut de Neurobiologie Alfred Fessard, Centre National de la Recherche Scientifique, Unité Propre de Recherche 9040, F91198 Gif sur Yvette Cedex, France

Published, JLR Papers in Press, April 16, 2005. DOI 10.1194/jlr.M500058-JLR200

¹ To whom correspondence should be addressed. e-mail: alain.brunelle{at}icsn.cnrs-gif.fr

Imaging with time-of-flight secondary ion mass spectrometry (TOF-SIMS) has expanded very rapidly with the development of gold cluster ion sources (Au₃⁺). It is now possible to acquire ion density maps (ion images) on a tissue section without any treatment and with a lateral resolution of few micrometers. In this article, we have taken advantage of this technique to study the degeneration/regeneration process in muscles of a Duchenne muscular dystrophy model mouse. Specific distribution of different lipid classes (fatty acids, triglycerides, phospholipids, tocopherol, coenzyme Q9, and cholesterol) allows us to distinguish three different regions on a mouse leg section: one is destroyed, another is degenerating (oxidative stress and deregulation of the phosphoinositol cycle), and the last one is stable.

TOF-SIMS imaging shows the ability to localize directly on a tissue section a great number of lipid compounds that reflect the state of the cellular metabolism.

Supplementary key words colocalization • oxidative stress • degeneration • destructured cells

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