HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M400498-JLR200v1 46/8/1652    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bojesen, I. N. Articles by Hansen, H. S. Search for Related Content PubMed PubMed Citation Articles by Bojesen, I. N. Articles by Hansen, H. S. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 46, 1652-1659, August 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

Membrane transport of anandamide through resealed human red blood cell membranes

Inge N. Bojesen^1,* and Harald S. Hansen

^* Department of Medical Biochemistry and Genetics, Laboratory B, University of Copenhagen, The Panum Institute, DK-2200 Copenhagen N, Denmark
Department of Pharmacology, Danish University of Pharmaceutical Sciences, DK-2100 Copenhagen Ø, Denmark

Published, JLR Papers in Press, June 1, 2005. DOI 10.1194/jlr.M400498-JLR200

¹ To whom correspondence should be addressed. e-mail: norby{at}imbg.ku.dk

The use of resealed red blood cell membranes (ghosts) allows the study of the transport of a compound in a nonmetabolizing system with a biological membrane. Transmembrane movements of anandamide (N-arachidonoylethanolamine, arachidonoylethanolamide) have been studied by exchange efflux experiments at 0°C and pH 7.3 with albumin-free and albumin-filled human red blood cell ghosts. The efflux kinetics is biexponential and is analyzed in terms of compartment models. The distribution of anandamide on the membrane inner to outer leaflet pools is determined to be 0.275 ± 0.023, and the rate constant of unidirectional flux from inside to outside is 0.361 ± 0.023 s^–1. The rate constant of unidirectional flux from the membrane to BSA in the medium ([BSA]_o) increases with the square root of [BSA]_o in accordance with the theory of an unstirred layer around ghosts. Anandamide passed through the red blood cell membrane very rapidly, within seconds. At a molar ratio of anandamide to BSA of <1, membrane binding of anandamide increases with increasing temperatures between 0°C and 37°C, and the equilibrium dissociation constants are in the nanomolar range.

The nature of membrane binding and the mechanism of membrane translocation are discussed.

Supplementary key words transmembrane movement • erythrocyte ghosts • membrane binding • exchange efflux

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Kaczocha, A. Hermann, S. T. Glaser, I. N. Bojesen, and D. G. Deutsch Anandamide Uptake Is Consistent with Rate-limited Diffusion and Is Regulated by the Degree of Its Hydrolysis by Fatty Acid Amide Hydrolase J. Biol. Chem., April 7, 2006; 281(14): 9066 - 9075. [Abstract] [Full Text] [PDF]

				I. N. Bojesen and H. S. Hansen Effect of an unstirred layer on the membrane permeability of anandamide J. Lipid Res., March 1, 2006; 47(3): 561 - 570. [Abstract] [Full Text] [PDF]