J. Lipid Res. Did you know there is a large type edition? Click here.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1194/jlr.M500060-JLR200 on May 1, 2005

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M500060-JLR200v1
46/8/1678    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, C.
Right arrow Articles by Dobrowsky, R. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, C.
Right arrow Articles by Dobrowsky, R. T.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Lipid Research, Vol. 46, 1678-1691, August 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

Ceramide displaces cholesterol from lipid rafts and decreases the association of the cholesterol binding protein caveolin-1

Cuijuan Yu, Michail Alterman and Rick T. Dobrowsky1

Department of Pharmacology and Toxicology, University of Kansas, Lawrence, KS 66045

Published, JLR Papers in Press, May 1, 2005. DOI 10.1194/jlr.M500060-JLR200

1 To whom correspondence should be addressed. e-mail: dobrowsky{at}ku.edu

Addition of exogenous ceramide causes a significant displacement of cholesterol in lipid raft model membranes. However, whether ceramide-induced cholesterol displacement is sufficient to alter the protein composition of caveolin-enriched lipid raft membranes is unknown. Therefore, we examined whether increasing endogenous ceramide levels with bacterial sphingomyelinase (bSMase) depleted cholesterol and changed the protein composition of caveolin-enriched membranes (CEMs) isolated from immortalized Schwann cells. bSMase increased ceramide levels severalfold and decreased the cholesterol content of detergent-insoluble CEMs by 25–50% within 2 h. To examine the effect of ceramide on the protein composition of the CEMs, we performed a quantitative proteomic analysis using stable isotope labeling of cells in culture and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Although ceramide rapidly depleted lipid raft cholesterol, the levels of the cholesterol binding protein caveolin-1 (Cav-1) decreased by 25% only after 8 h. Importantly, replenishing the cells with cholesterol rapidly reversed the loss of Cav-1 from the CEMs. Ceramide-induced cholesterol depletion increased the association of 5'-nucleotidase and ATP synthase ß-subunit with the CEMs but had a minimal effect on changing the abundance of other lipid raft proteins, such as flotillin-1 and G-proteins.

These results suggest that the ceramide-induced loss of cholesterol from CEMs may contribute to altering the lipid raft proteome.

Abbreviations: bSMase, bacterial sphingomyelinase; Cav-1, caveolin-1; CEM, caveolin-enriched membrane; dFCS, dialyzed fetal calf serum; MALDI-TOF MS, matrix-assisted laser desorption ionization time-of-flight mass spectrometry; MßCD, methyl-ß-cyclodextrin; SC, Schwann cell; SILAC, stable isotope labeling with amino acids in cell culture

Supplementary key words caveolae • proteomics • sphingolipid • Schwann cells • stable isotope labeling with amino acids in cell culture • SILAC


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
J. Neurosci.Home page
F. Galbiati, V. Basso, L. Cantuti, M. I. Givogri, A. Lopez-Rosas, N. Perez, C. Vasu, H. Cao, R. van Breemen, A. Mondino, et al.
Autonomic Denervation of Lymphoid Organs Leads to Epigenetic Immune Atrophy in a Mouse Model of Krabbe Disease
J. Neurosci., December 12, 2007; 27(50): 13730 - 13738.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
A. Rebillard, X. Tekpli, O. Meurette, O. Sergent, G. LeMoigne-Muller, L. Vernhet, M. Gorria, M. Chevanne, M. Christmann, B. Kaina, et al.
Cisplatin-Induced Apoptosis Involves Membrane Fluidification via Inhibition of NHE1 in Human Colon Cancer Cells
Cancer Res., August 15, 2007; 67(16): 7865 - 7874.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
N. E. Furland, G. M. Oresti, S. S. Antollini, A. Venturino, E. N. Maldonado, and M. I. Aveldano
Very Long-chain Polyunsaturated Fatty Acids Are the Major Acyl Groups of Sphingomyelins and Ceramides in the Head of Mammalian Spermatozoa
J. Biol. Chem., June 22, 2007; 282(25): 18151 - 18161.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
K. Krishnamurthy, S. Dasgupta, and E. Bieberich
Development and characterization of a novel anti-ceramide antibody
J. Lipid Res., April 1, 2007; 48(4): 968 - 975.
[Abstract] [Full Text] [PDF]


Home page
IOVSHome page
W. Chen, D. B. Jump, W. J. Esselman, and J. V. Busik
Inhibition of Cytokine Signaling in Human Retinal Endothelial Cells through Modification of Caveolae/Lipid Rafts by Docosahexaenoic Acid
Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 18 - 26.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
Megha, O. Bakht, and E. London
Cholesterol Precursors Stabilize Ordinary and Ceramide-rich Ordered Lipid Domains (Lipid Rafts) to Different Degrees: IMPLICATIONS FOR THE BLOCH HYPOTHESIS AND STEROL BIOSYNTHESIS DISORDERS
J. Biol. Chem., August 4, 2006; 281(31): 21903 - 21913.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Jean-Louis, S. Akare, M. A. Ali, E. A. Mash Jr., E. Meuillet, and J. D. Martinez
Deoxycholic Acid Induces Intracellular Signaling through Membrane Perturbations
J. Biol. Chem., May 26, 2006; 281(21): 14948 - 14960.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.