HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M500105-JLR200v1 46/8/1712    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kim, H. H. Articles by Chung, J. H. Search for Related Content PubMed PubMed Citation Articles by Kim, H. H. Articles by Chung, J. H. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 46, 1712-1720, August 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

Eicosapentaenoic acid inhibits UV-induced MMP-1 expression in human dermal fibroblasts

Hyeon Ho Kim^a,b,c, Chung Min Shin^a,b,c, Chi-Hyun Park^a,b,c, Kyu Han Kim^a,b,c, Kwang Hyun Cho^a,b,c, Hee Chul Eun^a,b,c and Jin Ho Chung^1,a,b,c

^a Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea
^b Laboratory of Cutaneous Aging Research, Clinical Research Institutes, Seoul National University Hospital, Seoul, Korea
^c Institute of Dermatological Science, Seoul National University, Seoul, Korea

Published, JLR Papers in Press, June 1, 2005. DOI 10.1194/jlr.M500105-JLR200

¹ To whom correspondence should be addressed. e-mail: jhchung{at}snu.ac.kr

Ultraviolet (UV) irradiation regulates UV-responsive genes, including matrix metalloproteinases (MMPs). Moreover, UV-induced MMPs cause connective tissue damage and the skin to become wrinkled and aged. Here, we investigated the effect of eicosapentaenoic acid (EPA), a dietary -3 fatty acid, on UV-induced MMP-1 expression in human dermal fibroblasts (HDFs). We found that UV radiation increases MMP-1 expression and that this is mediated by p44 and p42 MAP kinase (ERK) and Jun-N-terminal kinase (JNK) activation but not by p38 activation. Pretreatment of HDFs with EPA inhibited UV-induced MMP-1 expression in a dose-dependent manner and also inhibited the UV-induced activation of ERK and JNK by inhibiting ERK kinase (MEK1) and SAPK/ERK kinase 1 (SEK1) activation, respectively. Moreover, inhibition of ERK and JNK by EPA resulted in the decrease of c-Fos expression and c-Jun phosphorylation/expression induced by UV, respectively, which led to the inhibition of UV-induced activator protein-1 DNA binding activity. This inhibitory effect of EPA on MMP-1 was not mediated by an antioxidant effect. We also found that EPA inhibited 12-O-tetradecanoylphorbol-13-acetate- or tumor necrosis factor--induced MMP-1 expression in HDFs and UV-induced MMP-1 expression in HaCaT cells.

In conclusion, our results demonstrate that EPA can inhibit UV-induced MMP-1 expression by inhibiting the MEK1/ERK/c-Fos and SEK1/JNK/c-Jun pathways. Therefore, EPA is a potential agent for the prevention and treatment of skin aging.

Abbreviations: AA, arachidonic acid; AP-1, activator protein-1; COX-2, cyclooxygenase-2; DCF-DA, 2'-7'-dichlorofluorescein diacetate; DHA, docosahexaenoic acid; EMSA, electrophoretic mobility shift assay; EPA, eicosapentaenoic acid; ERK, p44 and p42 MAP kinase; JNK, Jun-N-terminal kinase; HDF, human dermal fibroblast; LA, linolenic acid; MAPK, mitogen-activated protein kinase; MEK1, MAP or ERK kinase; MMP-1, matrix metalloproteinase-1; NAC, N-acetyl cysteine; OA, oleic acid; PGE, prostaglandin E; PMSF, phenylmethylsulfonyl fluoride; ROS, reactive oxygen species; SEK, SAPK/ERK kinase; TNF-, tumor necrosis factor-; TPA, 12-O-tetradecanoylphorbol-13-acetate; UV, ultraviolet

Supplementary key words ultraviolet radiation • matrix metalloproteinase-1 • polyunsaturated fatty acid

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. Marion-Letellier, M. Butler, P. Dechelotte, R. J Playford, and S. Ghosh Comparison of cytokine modulation by natural peroxisome proliferator-activated receptor {gamma} ligands with synthetic ligands in intestinal-like Caco-2 cells and human dendritic cells--potential for dietary modulation of peroxisome proliferator-activated receptor {gamma} in intestinal inflammation Am. J. Clinical Nutrition, April 1, 2008; 87(4): 939 - 948. [Abstract] [Full Text] [PDF]

				C.-J. Liang, H. E. Ives, C.-M. Yang, and Y.-H. Ma 20-HETE inhibits the proliferation of vascular smooth muscle cells via transforming growth factor- J. Lipid Res., January 1, 2008; 49(1): 66 - 73. [Abstract] [Full Text] [PDF]

				N. Shimojo, S. Jesmin, S. Zaedi, S. Maeda, M. Soma, K. Aonuma, I. Yamaguchi, and T. Miyauchi Eicosapentaenoic acid prevents endothelin-1-induced cardiomyocyte hypertrophy in vitro through the suppression of TGF-beta1 and phosphorylated JNK Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H835 - H845. [Abstract] [Full Text] [PDF]

				H. H. Kim, S. Cho, S. Lee, K. H. Kim, K. H. Cho, H. C. Eun, and J. H. Chung Photoprotective and anti-skin-aging effects of eicosapentaenoic acid in human skin in vivo J. Lipid Res., May 1, 2006; 47(5): 921 - 930. [Abstract] [Full Text] [PDF]