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Journal of Lipid Research, Vol. 46, 2023-2028, September 2005
Copyright © 2005 by American Society for Biochemistry and Molecular Biology

Methods

Determining hepatic triglyceride production in mice: comparison of poloxamer 407 with Triton WR-1339

John S. Millar, Debra A. Cromley, Mary G. McCoy, Daniel J. Rader and Jeffrey T. Billheimer¹

Institute for Translational Medicine and Therapeutics and Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104

Published, JLR Papers in Press, July 1, 2005. DOI 10.1194/jlr.D500019-JLR200

¹ To whom correspondence should be addressed. e-mail: billheij{at}mail.med.upenn.edu

Triglyceride (TG), a water-insoluble energy-rich lipid, is secreted by the liver as part of very low density lipoproteins (VLDLs) to supply energy to extrahepatic tissues. Overproduction of VLDL is associated with increased risk of cardiovascular heart disease; this has renewed an interest in factors that affect hepatic TG production. The TG production rate is determined by measuring temporal increases in plasma TG under conditions in which TG hydrolysis by lipoprotein lipase (LPL) is inhibited. The nonionic detergent, Triton WR-1339 (Triton), has commonly been used to inhibit LPL for this purpose. Triton, in addition to inhibition of TG hydrolysis, has properties that have the potential to adversely influence lipoprotein metabolism. Another nonionic detergent, poloxamer 407 (P-407), also inhibits LPL.

In these studies, we demonstrate that P-407 is comparable to Triton in the determination of TG production but without the unwanted side effects of Triton.

Abbreviations: apoA-I, apolipoprotein A-I; FPLC, fast-protein liquid chromatography; HLB, hydrophilic/lipophilic balance; LPL, lipoprotein lipase; P-407, poloxamer 407; TG, triglyceride

Supplementary key words non-ionic detergents • hepatic lipids • lipoproteins

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