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Journal of Lipid Research, Vol. 47, 87-98, January 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

ADRP/adipophilin is degraded through the proteasome-dependent pathway during regression of lipid-storing cells

Yutaka Masuda^*, Hiroyuki Itabe^1,*,, Miho Odaki, Kotaro Hama, Yasuyuki Fujimoto, Masahiro Mori, Naoko Sasabe^*, Junken Aoki, Hiroyuki Arai and Tatsuya Takano

^* Department of Biological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University, 1091-1 Suarashi, Sagamiko, Tsukui, Kanagawa 199-0195, Japan
Department of Health Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033

Published, JLR Papers in Press, October 17, 2005.

¹ To whom correspondence should be addressed. e-mail: h-itabe{at}pharm.showa-u.ac.jp

Adipose differentiation-related protein (ADRP) is a major protein associated with lipid droplets in various types of cells, including macrophage-derived foam cells and liver cells. However, the role of ADRP in the processes of formation and regression of these cells is not understood. When J774 murine macrophages were incubated with either VLDL or oleic acid, their content of both ADRP and triacylglycerol (TG) increased 3- to 4-fold. Induction of ADRP during TG accumulation was also observed in oleic acid-treated HuH-7 human liver cells. Addition of triacsin C, a potent inhibitor of acyl-CoA synthase, for 6 h decreased the amount of TG in VLDL-induced foam cells and oleic acid-treated liver cells; it decreased the amount of ADRP protein in parallel, indicating the amount of ADRP reduced during regression of the lipid-storing cells. Addition of a proteasome inhibitor during triacsin C treatment abolished the ADRP decrease and accumulated polyubiquitinated ADRP. In addition, the proteasome inhibitor reversed not only the degradation of ADRP but also TG reduction by triacsin C. These results suggest that cellular amounts of ADRP and TG regulate each other and that the ubiquitin-proteasome system is involved in degradation of ADRP during regression of lipid-storing cells.

Supplementary key words lipid droplets • adipose differentiation-related protein • macrophages • liver cells • triacylglycerol • VLDL

Abbreviations: AcLDL, acetylated LDL; ADRP, adipose differentiation-related protein; ALP, alkaline phosphatase; FC, free cholesterol; LPDS, lipoprotein-deficient serum; LPS, lipopolysaccharide; mAb, monoclonal antibody; OxLDL, oxidized LDL; pAb, polyclonal antibody; PNS, postnuclear supernatant; TC, total cholesterol; TG, triacylglycerol

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