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Originally published In Press as doi:10.1194/jlr.M600095-JLR200 on August 2, 2006

Papers In Press, published online ahead of print October 1, 2006
J. Lipid Res., doi:10.1194/jlr.M600095-JLR200
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Journal of Lipid Research, Vol. 47, 2198-2207, October 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Microarray analysis indicates an important role for FABP5 and putative novel FABPs on a Western-type dietboxs

Menno Hoekstra1,2,*, Miranda Stitzinger1,*, Eva J. A. van Wanrooij*, Ingrid N. Michon*, J. Kar Kruijt*, J. Kamphorst{dagger}, M. Van Eck*, E. Vreugdenhil{dagger}, Theo J. C. Van Berkel* and Johan Kuiper*

* Division of Biopharmaceutics, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, 2300 RA Leiden, The Netherlands
{dagger} Division of Medical Pharmacology, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, 2300 RA Leiden, The Netherlands

boxs The online version of this article (available at http://www.jlr.org) contains additional two tables and one figure.

Published, JLR Papers in Press, August 2, 2006.

1 M. Hoekstra and M. Stitzinger contributed equally to this work.

2 To whom correspondence should be addressed. e-mail: hoekstra{at}lacdr.leidenuniv.nl

Liver parenchymal cells play a dominant role in hepatic metabolism and thereby total body cholesterol homeostasis. To gain insight into the specific pathways and genes involved in the response of liver parenchymal cells to increased dietary lipid levels under atherogenic conditions, changes in parenchymal cell gene expression upon feeding a Western-type diet for 0, 2, 4, and 6 weeks were determined using microarray analysis in LDL receptor-deficient mice, an established atherosclerotic animal model. Using ABI Mouse Genome Survey Arrays, we were able to detect 7,507 genes (28% of the total number on an array) that were expressed in parenchymal cells isolated from livers of LDL receptor-deficient mice at every time point investigated. Time-dependent gene expression profiling identified fatty acid binding protein 5 (FABP5) and four novel FABP5-like transcripts located on chromosomes 2, 8, and 18 as important proteins in the primary response of liver parenchymal cells to Western-type diet feeding, because their expression was 16- to 22-fold increased within the first 2 weeks on the Western-type diet. The rapid substantial increase in gene expression suggests that these FABPs may play an important role in the primary protection against the cellular toxicity of cholesterol, free fatty acids, and/or lipid oxidants. Furthermore, as a secondary response to the Western-type diet, liver parenchymal cells of LDL receptor-deficient mice stimulated glycolysis and lipogenesis pathways, resulting in a steady, more atherogenic serum lipoprotein profile (increased VLDL/LDL).

Supplementary key words liver parenchymal cell • gene expression • cholesterol diet • fatty acid binding proteins • time-dependent

Abbreviations: ACLY, ATP-citrate lyase; cRNA, complementary RNA; Ct, threshold cycle number; FABP, fatty acid binding protein; HPRT, hypoxanthine guanine phosphoribosyl transferase; PKLR, liver pyruvate kinase; SREBP, sterol-regulatory element binding protein; 36B4, acidic ribosomal phosphoprotein P0


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