J. Lipid Res.
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Originally published In Press as doi:10.1194/jlr.D600018-JLR200 on July 21, 2006

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Journal of Lipid Research, Vol. 47, 2340-2345, October 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Methods

Validation of the LC-MS/MS method for the quantification of mevalonic acid in human plasma and determination of the matrix effect

G. S. Saini*, T. A. Wani{dagger}, A. Gautam*, B. Varshney*, T. Ahmed*, K. S. Rajan*, K. K. Pillai{dagger} and J. K. Paliwal1,*

* Department of Metabolism and Pharmacokinetics, Ranbaxy Research Laboratories, Jamia Hamdard, Delhi, India
{dagger} Department of Pharmacology, Jamia Hamdard, Delhi, India

Published, JLR Papers in Press, July 21, 2006.

1 To whom correspondence should be addressed. e-mail: jyoti.paliwal{at}ranbaxy.com

A simple, specific, and sufficiently sensitive liquid chromatography-tandem mass spectrometry (negative-ion electrospray ionization) methodology to determine mevalonic acid (MVA) in human plasma is described, and its application to the analysis of rat plasma MVA levels after rosuvastatin administration is demonstrated. The method was validated over the linearity range of 0.5–50.0 ng/ml (r2 > 0.99) using deuterated MVA as an internal standard. The lower limit of quantification was 0.5 ng/ml. The assay procedure involved the isolation of MVA from plasma samples using solid-phase extraction. Chromatographic separation was achieved on a HyPurity Advance column with a mobile phase consisting of ammonium formate buffer (10 mM, pH 8.0) and acetonitrile (70:30, v/v). Excellent precision and accuracy were observed. MVA and deuterated mevalonolactone were stable in water and plasma under different storage and processing conditions. The recovery observed was low, which was attributable to a significant matrix effect. A significant decrease (30–40%; P < 0.05) was observed in rat plasma MVA levels after rosuvastatin administration.

Supplementary key words statin • biomarker • liquid chromatography-tandem mass spectrometry


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