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Journal of Lipid Research, Vol. 47, 2367-2373, November 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

* Membrane Regulation Section, Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-8028
Departments of Pediatrics and Obstetrics & Gynecology, Harbor-UCLA Medical Center Research and Education Institute, University of California at Los Angeles, Torrance, CA 90502
Published, JLR Papers in Press, August 25, 2006.
1 A. L. Magra and P. S. Mertz contributed equally to this work.
2 To whom correspondence should be addressed. e-mail: deanl{at}intra.niddk.nih.gov
Based on data developed with the use of isolated lipid droplets from neonatal rat lung lipofibroblasts, we speculated previously that the droplet coat protein, adipose differentiation-related protein (ADFP), mediated the transfer of lipids into type 2 lung epithelial cells for the production of surfactant phospholipids. The present studies were designed to test the role of ADFP in this transfer with the use of ADFP-coated lipid droplets from CHO fibroblast cells and a cultured human lung epithelial cell line. We found no role for ADFP in the lipid transfer and conclude that a lipase associated with the lipid droplets hydrolyzes their core triacylglycerols, releasing fatty acids that are taken up by the epithelial cells.
Supplementary key words lipid droplets surfactant phospholipid PAT proteins
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