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Journal of Lipid Research, Vol. 47, 341-348, February 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Transfer of very low density lipoprotein-associated phospholipids to activated human platelets

Salam Ibrahim, Anaël Djimet-Baboun, Valérie Pruneta-Deloche, Catherine Calzada, Michel Lagarde and Gabriel Ponsin¹

Unité Mixte de Recherche 585 Institut National de la Santé et de la Recherche Médicale/Institut National des Sciences Appliquées de Lyon-Lyon, Pathophysiology of Lipids and Membranes, Institut Multidisciplinaire de Biochimie des Lipides, Villeurbanne, France

Published, JLR Papers in Press, November 17, 2005.

¹ To whom correspondence should be addressed. e-mail: gabriel.ponsin{at}insa-lyon.fr

LDL-associated phospholipids (PLs) may be transferred into platelets. In this work, we characterized the role of VLDLs as PL donors. VLDL transferred radiolabeled PLs to platelets in a temperature- and concentration-dependent manner. LPL stimulated this process through its action on VLDL lipolysis, because it was abolished by tetrahydrolipstatin. LPL also stimulated the platelet production of thromboxane B₂ (TXB₂). Both LPL actions were inhibited in the presence of fatty acid-free albumin, suggesting that they were attributable to fatty acids generated during VLDL lipolysis. To study the relationship between PL transfers and platelet activation, we performed incubations in the presence of HDL, a physiological acceptor of PL released from VLDL. HDL antagonized the transfer of PL from VLDL to platelets but had no effect on the production of TXB₂, suggesting that PL transfers were driven by platelet activation. Confirming this idea, thrombin stimulated both the production of TXB₂ and the transfers of PL. In conclusion, VLDL can transfer PL to platelets. These transfers are stimulated by LPL and thrombin through their action on platelet activation. They might be enhanced in pathologies characterized by increased VLDL concentrations.

Supplementary key words lipoprotein lipase • thromboxane production • high density lipoprotein • tetrahydrolipstatin

Abbreviations: [³H]DPPC, 1,2-dipalmitoyl-([³H]methyl-choline)-phosphatidylcholine; FAF, fatty acid-free; [¹⁴C]PAPC, 1-palmitoyl-2-[1-¹⁴C]arachidonyl-phosphatidylcholine; [¹⁴C]PAPE, 1-palmitoyl-2-[1-¹⁴C]arachidonyl-phosphatidylethanolamine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PL, phospholipid; PLA₂, phospholipase A₂; TG, triglyceride; THL, tetrahydrolipstatin; TXB₂, thromboxane B₂

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