HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M500407-JLR200v1 47/3/553    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rodriguez-Cruz, M. Articles by Torres, N. Search for Related Content PubMed PubMed Citation Articles by Rodriguez-Cruz, M. Articles by Torres, N. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 47, 553-560, March 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Synthesis of long-chain polyunsaturated fatty acids in lactating mammary gland: role of 5 and 6 desaturases, SREBP-1, PPAR, and PGC-1

Maricela Rodriguez-Cruz^*,, Armando R. Tovar, Berenice Palacios-González, Martha del Prado^* and Nimbe Torres^1,

^* Unidad de Investigación Médica en Nutrición, Coordinación de Investigación en Salud, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, DF 06720 Mexico
Posgrado en Ciéncias Biológicas, Facultad de Medicina, UNAM, Mexico City, DF 04510, Mexico
Departamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, DF 14000 Mexico

Published, JLR Papers in Press, December 6, 2005.

¹ To whom correspondence should be addressed. e-mail: nimbet{at}quetzal.innsz.mx

The purpose of this work was to study whether rat lactating mammary gland can synthesize PUFAs through the expression of 5 and 6 desaturases (5D and 6D), whether these enzymes are regulated by the transcription factors sterol-regulatory element binding protein 1 (SREBP-1) and peroxisome proliferator-activated receptor (PPAR) and the coactivator peroxisome proliferator-activated receptor coactivator 1ß (PGC-1ß), and whether these desaturases are regulated by the lipid concentration in the diet. The results showed that on day 12 of lactation, 35% of the linoleic acid in the diet, which is the precursor of PUFAs, is transferred to the mammary gland. There was expression of 5D and 6D in mammary gland, and it was regulated by the corn oil content in the diet. The higher the corn oil content in the diet, the lower the expression of both desaturases. Induction of 5D and 6D was associated positively with similar changes in SREBP-1 and PGC-1ß. Expression of PPAR was barely detected and was not affected by the corn oil content in the diet, whereas PGC-1ß expression increased as the corn oil in the diet increased. These results indicate that the lactating mammary gland has the capacity to synthesize PUFAs and can be regulated by the lipid content in the diet.

Supplementary key words sterol-regulatory element binding protein 1 • peroxisome proliferator-activated receptor • peroxisome proliferator-activated receptor coactivator 1

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. Torres, C. J. Bautista, A. R. Tovar, G. Ordaz, M. Rodriguez-Cruz, V. Ortiz, O. Granados, P. W. Nathanielsz, F. Larrea, and E. Zambrano Protein restriction during pregnancy affects maternal liver lipid metabolism and fetal brain lipid composition in the rat Am J Physiol Endocrinol Metab, February 1, 2010; 298(2): E270 - E277. [Abstract] [Full Text] [PDF]

				A. Gutgesell, R. Ringseis, E. Schmidt, C. Brandsch, G. I Stangl, and K. Eder Downregulation of peroxisome proliferator-activated receptor {alpha} and its coactivators in liver and skeletal muscle mediates the metabolic adaptations during lactation in mice J. Mol. Endocrinol., December 1, 2009; 43(6): 241 - 250. [Abstract] [Full Text] [PDF]

				A. Gutgesell, R. Ringseis, and K. Eder Short communication: Dietary conjugated linoleic acid down-regulates fatty acid transporters in the mammary glands of lactating rats J Dairy Sci, March 1, 2009; 92(3): 1169 - 1173. [Abstract] [Full Text] [PDF]

				S. Fourcade, M. Ruiz, C. Camps, A. Schluter, S. M. Houten, P. A. W. Mooyer, T. Pampols, G. Dacremont, R. J. A. Wanders, M. Giros, et al. A key role for the peroxisomal ABCD2 transporter in fatty acid homeostasis Am J Physiol Endocrinol Metab, January 1, 2009; 296(1): E211 - E221. [Abstract] [Full Text] [PDF]

				L. Xie and S. M. Innis Genetic Variants of the FADS1 FADS2 Gene Cluster Are Associated with Altered (n-6) and (n-3) Essential Fatty Acids in Plasma and Erythrocyte Phospholipids in Women during Pregnancy and in Breast Milk during Lactation J. Nutr., November 1, 2008; 138(11): 2222 - 2228. [Abstract] [Full Text] [PDF]

				C. M. R. LeMoine, C. E. Genge, and C. D. Moyes Role of the PGC-1 family in the metabolic adaptation of goldfish to diet and temperature J. Exp. Biol., May 1, 2008; 211(9): 1448 - 1455. [Abstract] [Full Text] [PDF]

				R. Ringseis, C. Dathe, A. Muschick, C. Brandsch, and K. Eder Oxidized Fat Reduces Milk Triacylglycerol Concentrations by Inhibiting Gene Expression of Lipoprotein Lipase and Fatty Acid Transporters in the Mammary Gland of Rats J. Nutr., September 1, 2007; 137(9): 2056 - 2061. [Abstract] [Full Text] [PDF]