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Papers In Press, published online ahead of print May 1, 2006
J. Lipid Res., doi:10.1194/jlr.M500462-JLR200

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Journal of Lipid Research, Vol. 47, 1005-1013, May 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Lipophorin receptor of Bombyx mori: cDNA cloning, genomic structure, alternative splicing, and isolation of a new isoform^1,

Ravikumar Gopalapillai², Keiko Kadono-Okuda, Kozo Tsuchida, Kimiko Yamamoto, Junko Nohata, Masahiro Ajimura and Kazuei Mita³

Insect Genome Laboratory, National Institute of Agrobiological Sciences, Owashi 1-2, Tsukuba, Japan 305 8634

¹ The sequences mentioned in this article have been deposited in the Data Bank of Japan (accession numbers AB201471, AB201472, AB201473, and AB201474).

The online version of this article (availabe at http://www.jlr.org) contains additional table and figure.

Published, JLR Papers in Press, February 15, 2006.

² Present address of R. Gopalapillai: Seri-Biotech Research Laboratory, Central Silk Board, Carmelaram Post, Kodathi, Bangalore 560 035, India.

³ To whom correspondence should be addressed. e-mail: kmita{at}nias.affrc.go.jp

The cDNA and genomic structure of a putative lipophorin receptor from the silkworm, Bombyx mori (BmLpR), indicated the presence of four isoforms, designated LpR1, LpR2, LpR3, and LpR4. The deduced amino acid sequence of each isoform showed five functional domains that are homologous to vertebrate very low density lipoprotein receptor (VLDLR). All four isoforms seem to have originated from a single gene by alternative splicing and were differentially expressed in a tissue- and stage-specific manner. BmLpR1 harbored an additional 27 amino acids in the O-linked sugar domain, resulting in an extra exon. The silkworm BmLpR gene consisted of 16 exons separated by 15 introns spanning >122 kb and was at least three times larger than the human VLDLR gene. Surprisingly, one of the isoforms, LpR4, was expressed specifically in the brain and central nervous system. Additionally, it had a unique cytoplasmic tail, leading to the proposition that it represents a new candidate LpR for possible brain-related function(s). This is the first report on the genomic characterization of an arthropod lipoprotein receptor gene and the identification of a brain-specific receptor variant from a core member of the low density lipoprotein receptor family in invertebrates.

Abbreviations: BAC, bacterial artificial chromosome; EGF, epidermal growth factor; HDLp, high density lipophorin; LBD, ligand binding domain; LDLR, low density lipoprotein receptor; Lp, lipophorin; LpR, lipophorin receptor; LRP, low density lipoprotein receptor-related protein; RACE, rapid amplification of cDNA ends; UTR, untranslated region; VLDLR, very low density lipoprotein receptor

Supplementary key words silkworm • lipoprotein receptor • isoforms

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