J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1194/jlr.M500447-JLR200 on March 13, 2006

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M500447-JLR200v1
47/6/1168    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rodriguez-Agudo, D.
Right arrow Articles by Pandak, W. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rodriguez-Agudo, D.
Right arrow Articles by Pandak, W. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 47, 1168-1175, June 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Localization of StarD5 cholesterol binding protein

Daniel Rodriguez-Agudo*, Shunlin Ren*, Phillip B. Hylemon{dagger}, Raul Montañez§, Kaye Redford*, Ramesh Natarajan*, Miguel Angel Medina§, Gregorio Gil** and William M. Pandak1,*

* Department of Medicine, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA
{dagger} Department of Microbiology/Immunology, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA
** Department of Biochemistry, Veterans Affairs Medical Center and Virginia Commonwealth University, Richmond, VA
§ Departamento de Biologia Molecular y Bioquimica, Universidad de Malaga, Malaga, Spain

Published, JLR Papers in Press, March 13, 2006.

1 To whom correspondence should be addressed. e-mail: wmpandak{at}hsc.vcu.edu

Human StarD5 belongs to the StarD4 subfamily of START (for steroidogenic acute regulatory lipid transfer) domain proteins. We previously reported that StarD5 is located in the cytosolic fraction of human liver and binds cholesterol and 25-hydroxycholesterol. After overexpression of the gene encoding StarD5 in primary rat hepatocytes, free cholesterol accumulated in intracellular membranes. These findings suggested StarD5 to be a directional cytosolic sterol transporter. The objective of this study was to determine the localization of StarD5 in human liver. Western blot analysis confirmed StarD5's presence in the liver but not in human hepatocytes. Immunohistochemistry studies showed StarD5 localized within sinusoidal lining cells in the human liver and colocalized with CD68, a marker for Kupffer cells. Western blot analyses identified the presence of StarD5 in monocytes and macrophages as well as mast cells, basophils, and promyelocytic cells, but not in human hepatocytes, endothelial cells, fibroblasts, osteocytes, astrocytes, or brain tissue. Cell fractionation and immunocytochemistry studies on THP-1 macrophages localized StarD5 to the cytosol and supported an association with the Golgi. The presence of this cholesterol/25-hydroxycholesterol-binding protein in cells related to inflammatory processes provides new clues to the role of this protein in free sterol transport in the cells and in lipid-mediated atherogenesis.

Supplementary key words liver • Kupffer cells • macrophages • sterol transporter


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
D. Rodriguez-Agudo, S. Ren, E. Wong, D. Marques, K. Redford, G. Gil, P. Hylemon, and W. M. Pandak
Intracellular cholesterol transporter StarD4 binds free cholesterol and increases cholesteryl ester formation
J. Lipid Res., July 1, 2008; 49(7): 1409 - 1419.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
W. L. Miller
StAR Search--What We Know about How the Steroidogenic Acute Regulatory Protein Mediates Mitochondrial Cholesterol Import
Mol. Endocrinol., March 1, 2007; 21(3): 589 - 601.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
W.-J. Wang, J. M. Baez, R. Maurer, H. M. Dansky, and D. E. Cohen
Homozygous disruption of Pctp modulates atherosclerosis in apolipoprotein E-deficient mice
J. Lipid Res., November 1, 2006; 47(11): 2400 - 2407.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.