Advertisement
J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1194/jlr.M500507-JLR200 on March 7, 2006

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M500507-JLR200v1
47/6/1227    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ma, F. X.
Right arrow Articles by Han, Z. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ma, F. X.
Right arrow Articles by Han, Z. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 47, 1227-1237, June 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Oxidized low density lipoprotein impairs endothelial progenitor cells by regulation of endothelial nitric oxide synthase

Feng Xia Ma*, Bin Zhou*, Zhong Chen*, Qian Ren*,{dagger}, Shi Hong Lu*,{dagger}, Tatsuya Sawamura§ and Zhong Chao Han1,*,{dagger}

* State Key Laboratory of Experimental Hematology and National Research Center for Stem Cell Engineering and Technology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, People's Republic of China
{dagger} AmCellGene Co., Ltd., Tianjin Economic and Technological Development Area Center of Life Science and Technology, Tianjin, People's Republic of China
§ National Cardiovascular Center Research Institute, Suita, Osaka, Japan

Published, JLR Papers in Press, March 7, 2006.

1 To whom correspondence should be addressed. e-mail: tihzchan{at}public.tpt.tj.cn

Oxidized low density lipoprotein (OxLDL) is one of the most important risk factors of cardiovascular disease. Here, we study the impact of OxLDL on endothelial progenitor cells (EPCs) and determine whether OxLDL affects EPCs by an inhibitory effect on endothelial nitric oxide synthase (eNOS). It was found that OxLDL decreased EPC survival and impaired its adhesive, migratory, and tube-formation capacities in a dose-dependent manner. However, all of the detrimental effects of OxLDL were attenuated by pretreatment of EPCs with lectin-like oxidized low density lipoprotein receptor (LOX-1) monoclonal antibody or L-arginine. Western blot analysis revealed that OxLDL dose-dependently decreased Akt phosphorylation and eNOS protein expression and increased LOX-1 protein expression. Furthermore, OxLDL caused a decrease in eNOS mRNA expression and an increase in LOX-1 mRNA expression. These data indicate that OxLDL inhibits EPC survival and impairs its function, and this action is attributable to an inhibitory effect on eNOS.

Supplementary key words angiogenesis • stem cell • apoptosis • Akt

Abbreviations: Dil Ac-LDL, 1,1'-dioctadecyl-3,3,3'3' -tetramethylindocar-bocyanine perchlorate (Dil)-labeled acetylated low density lipoprotein; eNOS, endothelial nitric oxide synthase; EPC, endothelial progenitor cell; HUVEC, human umbilical vein endothelial cell; L-NAME, N-nitro-L-arginine methyl ester; LOX-1, lectin-like oxidized low density lipoprotein receptor; LOX-1 MAb, lectin-like oxidized low density lipoprotein receptor monoclonal antibody; MNC, mononuclear cell; NO, nitric oxide; OxLDL, oxidized low density lipoprotein; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; UEA-1, Ulex europaeus agglutinin-1; VEGF, vascular endothelial growth factor


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Ther Adv Cardiovasc DisHome page
M. Pirro, F. Bagaglia, L. Paoletti, R. Razzi, and M. R. Mannarino
Review: Hypercholesterolemia-associated endothelial progenitor cell dysfunction
Therapeutic Advances in Cardiovascular Disease, October 1, 2008; 2(5): 329 - 339.
[Abstract] [PDF]

Home page
 J. Lipid Res.Home page
D. Tang, J. Lu, J. P. Walterscheid, H.-H. Chen, D. A. Engler, T. Sawamura, P.-Y. Chang, H. J. Safi, C.-Y. Yang, and C.-H. Chen
Electronegative LDL circulating in smokers impairs endothelial progenitor cell differentiation by inhibiting Akt phosphorylation via LOX-1
J. Lipid Res., January 1, 2008; 49(1): 33 - 47.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. Cheng, R. Cui, C.-H. Chen, and J. Du
Oxidized Low-Density Lipoprotein Stimulates p53-Dependent Activation of Proapoptotic Bax Leading to Apoptosis of Differentiated Endothelial Progenitor Cells
Endocrinology, May 1, 2007; 148(5): 2085 - 2094.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
V. L.T. Ballard and J. M. Edelberg
Stem Cells and the Regeneration of the Aging Cardiovascular System
Circ. Res., April 27, 2007; 100(8): 1116 - 1127.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
B. Zhou, F. X. Ma, P. X. Liu, Z. H. Fang, S. L. Wang, Z. B. Han, M.-C. Poon, and Z. C. Han
Impaired therapeutic vasculogenesis by transplantation of OxLDL-treated endothelial progenitor cells
J. Lipid Res., March 1, 2007; 48(3): 518 - 527.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement