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Originally published In Press as doi:10.1194/jlr.M500512-JLR200 on March 27, 2006

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Journal of Lipid Research, Vol. 47, 1250-1260, June 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Assessing the effects of LXR agonists on cellular cholesterol handling: a stable isotope tracer study

Karpagam Aravindhan*, Christine L. Webb{dagger}, Michael Jaye{dagger}, Avijit Ghosh*, Robert N. Willette{dagger}, N. John DiNardo* and Beat M. Jucker1,{dagger}

* Department of Applied Physics, College of Arts and Sciences, Drexel University, Philadelphia, PA 19104
{dagger} Cardiovascular and Urogenital Center of Excellence in Drug Discovery, GlaxoSmithKline, King of Prussia, PA 19406

Published, JLR Papers in Press, March 27, 2006.

1 To whom correspondence should be addressed. e-mail: beat_m_jucker{at}gsk.com

The liver X receptors (LXRs) {alpha} and ß are responsible for the transcriptional regulation of a number of genes involved in cholesterol efflux from cells and therefore may be molecular targets for the treatment of cardiovascular disease. However, the effects of LXR ligands on cholesterol turnover in cells has not been examined comprehensively. In this study, cellular cholesterol handling (e.g., synthesis, catabolism, influx, and efflux) was examined using a stable isotope labeling study and a two-compartment modeling scheme. In HepG2 cells, the incorporation of 13C into cholesterol from [1-13C]acetate was analyzed by mass isotopomer distribution analysis in conjunction with nonsteady state, multicompartment kinetic analysis to calculate the cholesterol fluxes. Incubation with synthetic, nonsteroidal LXR agonists (GW3965, T0901317, and SB742881) increased cholesterol synthesis (~10-fold), decreased cellular cholesterol influx (71–82%), and increased cellular cholesterol efflux (1.7- to 1.9-fold) by 96 h. As a consequence of these altered cholesterol fluxes, cellular cholesterol decreased (36–39%) by 96 h. The increased cellular cholesterol turnover was associated with increased expression of the LXR-activated genes ABCA1, ABCG1, FAS, and sterol-regulatory element binding protein 1c. In summary, the mathematical model presented allows time-dependent calculations of cellular cholesterol fluxes. These data demonstrate that all of the cellular cholesterol fluxes were altered by LXR activation and that the increase in cholesterol synthesis did not compensate for the increased cellular cholesterol efflux, resulting in a net cellular cholesterol loss.

Supplementary key words liver X receptor • HepG2 • turnover • compartmental • mass isotopomer distribution analysis

Abbreviations: APE, atom percentage excess; apoB, apolipoprotein B; CYP7A1, cholesterol 7{alpha}-hydroxylase; DMHCA, N,N-dimethly-3ß-hydroxy-cholenamide; LDLR, low density lipoprotein receptor; LXR, liver X receptor; MIDA, mass isotopomer distribution analysis; SR-BI, scavenger receptor class B type I; SREBP1c, sterol-regulatory element binding protein 1c


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