HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M600145-JLR200v1 47/7/1542    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Abe-Dohmae, S. Articles by Yokoyama, S. Search for Related Content PubMed PubMed Citation Articles by Abe-Dohmae, S. Articles by Yokoyama, S. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 47, 1542-1550, July 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Serum amyloid A generates high density lipoprotein with cellular lipid in an ABCA1- or ABCA7-dependent manner

Sumiko Abe-Dohmae^*, Koichi H. Kato, Yoshitaka Kumon, Wei Hu^*, Hideaki Ishigami^*, Noriyuki Iwamoto^*, Mitsuyo Okazaki^**, Chen-Ai Wu^*, Maki Tsujita^*, Kazumitsu Ueda and Shinji Yokoyama^1,*

^* Biochemistry, Cell Biology, and Metabolism, Nagoya City University Graduate School of Medical Sciences, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan
Life Science Department, Nagoya City University Graduate School of Natural Sciences, Mizuho-cho, Mizuho-ku, Nagoya 467-8501, Japan
Department of Laboratory Medicine, Kochi University Medical School, Kohasu Okoh-cho, Nankoku, Kochi 783-8505, Japan
^** Laboratory of Chemistry, College of Liberal Arts and Science, Tokyo Medical and Dental University, Ichikawa 272-0827, Japan
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan

Published, JLR Papers in Press, April 10, 2006.

¹ To whom correspondence should be addressed. e-mail: syokoyam{at}med.nagoya-cu.ac.jp

Serum amyloid A (SAA) is an amphiphilic helical protein that is found associated with plasma HDL in various pathological conditions, such as acute or chronic inflammation. Cellular lipid release and generation of HDL by this protein were investigated, in comparison with the reactions by apolipoprotein A-I (apoA-I) and several types of cells that appear with various specific profiles of cholesterol and phospholipid release. SAA mediated cellular lipid release from these cells with the same profile as apoA-I. Upregulation of cellular ABCA1 protein by liver X receptor/retinoid X receptor agonists resulted in an increase of cellular lipid release by apoA-I and SAA. SAA reacted with the HEK293-derived clones that stably express human ABCA1 (293/2c) or ABCA7 (293/6c) to generate cholesterol-containing HDL in a similar manner to apoA-I. Dibutyryl cyclic AMP and phorbol 12-myristate 13-acetate, which differentiate apoA-I-mediated cellular lipid release between 293/2c and 293/6c, also exhibited the same differential effects on the SAA-mediated reactions. No evidence was found for the ABCA1/ABCA7-independent lipid release by SAA. Characterization of physicochemical properties of the HDL revealed that SAA-generated HDL particles had higher density, larger diameter, and slower electrophoretic mobility than those generated by apoA-I. These results demonstrate that SAA generates cholesterol-containing HDL directly with cellular lipid and that the reaction is mediated by ABCA1 and ABCA7.

Abbreviations: apoA-I, apolipoprotein A-I; dBcAMP, dibutyryl cyclic AMP; DF medium, 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium; D-PBS, Dulbecco's phosphate-buffered saline; IL, interleukin; LPS, lipopolysaccharide; PMA, phorbol 12-myristate 13-acetate; SAA, serum amyloid A; SR-BI, scavenger receptor class B type I; TNF-, tumor necrosis factor-; TNFR-II, tumor necrosis factor receptor-II

Supplementary key words apolipoprotein • cholesterol • ATP binding cassette transporter A1 • ATP binding cassette transporter A7

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				W. Hu, S. Abe-Dohmae, M. Tsujita, N. Iwamoto, O. Ogikubo, T. Otsuka, Y. Kumon, and S. Yokoyama Biogenesis of HDL by SAA is dependent on ABCA1 in the liver in vivo J. Lipid Res., February 1, 2008; 49(2): 386 - 393. [Abstract] [Full Text] [PDF]

				M. Westerterp, J. F.P. Berbee, N. M.M. Pires, G. J.D. van Mierlo, R. Kleemann, J. A. Romijn, L. M. Havekes, and P. C.N. Rensen Apolipoprotein C-I Is Crucially Involved in Lipopolysaccharide-Induced Atherosclerosis Development in Apolipoprotein E Knockout Mice Circulation, November 6, 2007; 116(19): 2173 - 2181. [Abstract] [Full Text] [PDF]

				M. Heller, E. Schlappritzi, D. Stalder, J.-M. Nuoffer, and A. Haeberli Compositional Protein Analysis of High Density Lipoproteins in Hypercholesterolemia by Shotgun LC-MS/MS and Probabilistic Peptide Scoring Mol. Cell. Proteomics, June 1, 2007; 6(6): 1059 - 1072. [Abstract] [Full Text] [PDF]

				N. Iwamoto, S. Abe-Dohmae, R. Sato, and S. Yokoyama ABCA7 expression is regulated by cellular cholesterol through the SREBP2 pathway and associated with phagocytosis J. Lipid Res., September 1, 2006; 47(9): 1915 - 1927. [Abstract] [Full Text] [PDF]