HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: D600010-JLR200v1 47/7/1572    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nakano, Y. Articles by Tobe, T. Search for Related Content PubMed PubMed Citation Articles by Nakano, Y. Articles by Tobe, T. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 47, 1572-1582, July 2006
Copyright © 2006 by American Society for Biochemistry and Molecular Biology

Methods

A novel enzyme-linked immunosorbent assay specific for high-molecular-weight adiponectin

Yasuko Nakano^1,*, Sachiko Tajima^*, Ai Yoshimi^*, Haruyo Akiyama, Motoo Tsushima, Toshihiro Tanioka^*, Takaharu Negoro^*, Motowo Tomita and Takashi Tobe^*

^* Department of Medicinal Information, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
International University of Health and Welfare, 13-1 Higashikaigan-cyo, Atami-shi, Shizuoka 413-0012, Japan, and Department of Internal Medicine, Keio University, School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan

Published, JLR Papers in Press, April 7, 2006.

¹ To whom correspondence should be addressed. e-mail: yanakano{at}pharm.showa-u.ac.jp

Human plasma contains at least three forms of adiponectin: a trimer, a hexamer, and a high-molecular-weight (HMW) multimer. We purified HMW adiponectin from human plasma using its affinity to gelatin and obtained monoclonal antibodies against it. On Western blot analysis, the reactivity of these monoclonal antibodies was shown to be restricted to a non-heat-denatured form of adiponectin molecules. On heating, the collagen-like domain of adiponectin molecules became denatured, and thus the trimer form could not be maintained. From these, monoclonal antibodies against HMW adiponectin were suggested to react with the intact trimer of adiponectin. With these monoclonal antibodies, we developed a sandwich ELISA system for quantifying adiponectin in human serum. Its specificity was verified by analysis of serum fractions separated by gel-filtration chromatography, and our ELISA system was found to be HMW adiponectin-specific. With this novel ELISA, the HMW adiponectin concentrations were 8.4 ± 5.5 µg/ml (mean ± SD) in healthy women and 6.2 ± 3.6 µg/ml in healthy men. Also, serum with a lower HMW adiponectin concentration was shown to have a lower HMW ratio (i.e., HMW adiponectin/total adiponectin).

Supplementary key words high-molecular-weight multimer • monoclonal antibody • gelatin-binding protein of 28 kDa

Abbreviations: CRP, C-reactive protein; GBP28, gelatin binding protein of 28 kDa; HMW, high-molecular-weight; POD-IH7, horseradish peroxidase-conjugated IH7 Fab'

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. Liu, T. Schuster, M. Baumann, M. Roos, D. Sollinger, J. Lutz, U. Heemann, and M. von Eynatten Comparison of Immunoassays for the Selective Measurement of Human High-Molecular Weight Adiponectin Clin. Chem., March 1, 2009; 55(3): 568 - 572. [Abstract] [Full Text] [PDF]

				J.-P. Empana Adiponectin isoforms and cardiovascular disease: the epidemiological evidence has just begun Eur. Heart J., May 2, 2008; 29(10): 1221 - 1223. [Full Text] [PDF]

				T. Tanaka, T. Tsutamoto, H. Sakai, K. Nishiyama, M. Fujii, T. Yamamoto, and M. Horie Effect of atrial natriuretic peptide on adiponectin in patients with heart failure Eur J Heart Fail, April 1, 2008; 10(4): 360 - 366. [Abstract] [Full Text] [PDF]

				P. G. Cammisotto, I. Londono, D. Gingras, and M. Bendayan Control of glycogen synthase through ADIPOR1-AMPK pathway in renal distal tubules of normal and diabetic rats Am J Physiol Renal Physiol, April 1, 2008; 294(4): F881 - F889. [Abstract] [Full Text] [PDF]

				Y. Tabara, H. Osawa, R. Kawamoto, R. Tachibana-Iimori, M. Yamamoto, J. Nakura, T. Miki, H. Makino, and K. Kohara Reduced High-Molecular-Weight Adiponectin and Elevated High-Sensitivity C-Reactive Protein Are Synergistic Risk Factors for Metabolic Syndrome in a Large-Scale Middle-Aged to Elderly Population: the Shimanami Health Promoting Program Study J. Clin. Endocrinol. Metab., March 1, 2008; 93(3): 715 - 722. [Abstract] [Full Text] [PDF]

				M. Fasshauer, T. Waldeyer, J. Seeger, S. Schrey, T. Ebert, J. Kratzsch, U. Lossner, M. Bluher, M. Stumvoll, R. Faber, et al. Circulating high-molecular-weight adiponectin is upregulated in preeclampsia and is related to insulin sensitivity and renal function Eur. J. Endocrinol., February 1, 2008; 158(2): 197 - 201. [Abstract] [Full Text] [PDF]

				M. K. Sinha, T. Songer, Q. Xiao, J. H. Sloan, J. Wang, S. Ji, W. E. Alborn, R. A. Davis, M. M. Swarbrick, K. L. Stanhope, et al. Analytical Validation and Biological Evaluation of a High Molecular-Weight Adiponectin ELISA Clin. Chem., December 1, 2007; 53(12): 2144 - 2151. [Abstract] [Full Text] [PDF]

				J. Lo, L. E. Bernstein, B. Canavan, M. Torriani, M. B. Jackson, R. S. Ahima, and S. K. Grinspoon Effects of TNF-{alpha} neutralization on adipocytokines and skeletal muscle adiposity in the metabolic syndrome Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E102 - E109. [Abstract] [Full Text] [PDF]

				K. K. Andersen, J. Frystyk, O. D. Wolthers, C. Heuck, and A. Flyvbjerg Gender Differences of Oligomers and Total Adiponectin during Puberty: A Cross-Sectional Study of 859 Danish School Children J. Clin. Endocrinol. Metab., May 1, 2007; 92(5): 1857 - 1862. [Abstract] [Full Text] [PDF]

				A. Korner, K. Pazaitou-Panayiotou, T. Kelesidis, I. Kelesidis, C. J. Williams, A. Kaprara, J. Bullen, A. Neuwirth, S. Tseleni, N. Mitsiades, et al. Total and High-Molecular-Weight Adiponectin in Breast Cancer: In Vitro and in Vivo Studies J. Clin. Endocrinol. Metab., March 1, 2007; 92(3): 1041 - 1048. [Abstract] [Full Text] [PDF]

				M. E. Trujillo and P. E. Scherer Adipose Tissue-Derived Factors: Impact on Health and Disease Endocr. Rev., December 1, 2006; 27(7): 762 - 778. [Abstract] [Full Text] [PDF]

				R. Nakashima, N. Kamei, K. Yamane, S. Nakanishi, A. Nakashima, and N. Kohno Decreased Total and High Molecular Weight Adiponectin Are Independent Risk Factors for the Development of Type 2 Diabetes in Japanese-Americans J. Clin. Endocrinol. Metab., October 1, 2006; 91(10): 3873 - 3877. [Abstract] [Full Text] [PDF]

				N. Sattar, G. Wannamethee, N. Sarwar, J. Tchernova, L. Cherry, A. M. Wallace, J. Danesh, and P. H. Whincup Adiponectin and Coronary Heart Disease: A Prospective Study and Meta-Analysis Circulation, August 15, 2006; 114(7): 623 - 629. [Abstract] [Full Text] [PDF]