J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Originally published In Press as doi:10.1194/jlr.M600365-JLR200 on October 30, 2006

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Journal of Lipid Research, Vol. 48, 127-138, January 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Immunosuppressive effects of polyunsaturated fatty acids on antigen presentation by human leukocyte antigen class I molecules

Saame Raza Shaikh1 and Michael Edidin

Department of Biology, Johns Hopkins University, Baltimore, MD 21218

Published, JLR Papers in Press, October 30, 2006.

1 To whom correspondence should be addressed. e-mail: saameshaikh{at}gmail.com

Dietary supplementation with polyunsaturated fatty acids (PUFAs) has immunosuppressive effects; however, the molecular targets of PUFAs and their mode of action remain unclear. One possible target is antigen presentation to T cells through the human leukocyte antigen (HLA) class I pathway. Here we show that incorporation of PUFAs lowers target cell susceptibility to lysis by effector T cells. Treatment of B lymphoblast targets with the {omega}-6 PUFA arachidonic acid (AA) or {omega}-3 docosahexaenoic acid lowered their susceptibility to lysis by alloreactive CD8+ T cells by ~20–25%. HLA class I surface levels and their rate of endoplasmic reticulum (ER)-Golgi traffic were also reduced by PUFA treatment. Calibration experiments showed that the ~15% reduction in surface HLA I was not sufficient to completely account for the decreased lysis. However, PUFAs significantly lowered antigen-presenting cell-T cell conjugate formation, by ~30–40%. Taken together, our data show for the first time that an {omega}-6 and an {omega}-3 PUFA affect the HLA class I pathway of B lymphoblasts. Our findings suggest that elimination of self- and pathogen-derived peptides by effectors may be compromised by dietary PUFA supplementation. In addition, PUFA-mediated changes in ER-Golgi trafficking point to a new area of PUFA modulation of immune responses.

Supplementary key words arachidonic acid • cytolysis • docosahexaenoic acid • membrane microviscosity • surface expression • trafficking

Abbreviations: AA, arachidonic acid; APC, antigen-presenting cell; BFA, Brefeldin A; BHT, butylated hydroxytoluene; CTL, cytotoxic T lymphocyte; DHA, docosahexaenoic acid; ER, endoplasmic reticulum; FACS, fluorescence-activated cell sorting; HBSS, Hanks' balanced salt solution; HLA, human leukocyte antigen; NL, neutral lipid; PA, palmitic acid; PC, phosphatidylcholine; PUFA, polyunsaturated fatty acid; PVP, polyvinylpyrollidone; TCR, T cell receptor; 7-AAD, 7-amino-actinomycin D


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