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Journal of Lipid Research, Vol. 48, 235-241, January 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Methods

Rapid characterization of the fatty acyl composition of complex lipids by collision-induced dissociation time-of-flight mass spectrometry

Steven Wynn Esch^*, Pamela Tamura^*, Alexis A. Sparks^*, Mary R. Roth^*, Shivakumar P. Devaiah, Ernst Heinz, Xuemin Wang, Todd D. Williams^** and Ruth Welti^1,*

^* Kansas Lipidomics Research Center, Division of Biology, Kansas State University, Manhattan, KS 66506
Department of Biology, University of Missouri-St. Louis and Danforth Plant Science Center, St. Louis, MO 63121
Biozentrum Klein Flottbeck, University of Hamburg, D-22609 Hamburg, Germany
^** University of Kansas Mass Spectrometry Laboratory, University of Kansas, Lawrence, KS 66045

Published, JLR Papers in Press, October 19, 2006.

¹ To whom correspondence should be addressed. e-mail: welti{at}ksu.edu

Profiling of leaf extracts from mutants of Arabidopsis with defects in lipid desaturation demonstrates the utility of collision-induced dissociation time-of-flight mass spectrometry (CID-TOF MS) for screening biological samples for fatty acid compositional alterations. CID-TOF MS uses the collision cell of a quadrupole time-of-flight mass spectrometer to simultaneously fragment all of the ions produced by an ionization source. Electrospray ionization CID-TOF MS in the negative mode can be used to analyze fatty acyl anions derived from complex lipids as well as free fatty acids. Although acyl anion yield is shown to be a function of the lipid class and the position on the glycerol backbone, acyl compositional profiles can be determined, and the TOF detector provides resolution of nominally isobaric acyl species in the profiles. Good precision is obtained when data are acquired for 1 min per sample.

Supplementary key words phospholipid • galactolipid • oxylipins • lipidomics

Abbreviations: CID-TOF MS, collision-induced dissociation time-of-flight mass spectrometry; di15:0 PE, 1-15:0,2-15:0 phosphatidylethanolamine; ESI, electrospray ionization; FID, flame ionization detection; MGDG, monogalactosyldiacylglycerol; OPPC, 1-oleoyl (18:1),2-palmitoyl (16:0) phosphatidylcholine; POPC, 1-palmitoyl (16:0),2-oleoyl (18:1) phosphatidylcholine; POPE, 1-palmitoyl (16:0),2-oleoyl (18:1) phosphatidylethanolamine; Q-TOF, quadrupole time-of-flight

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