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Journal of Lipid Research, Vol. 48, 2334-2343, November 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Theaflavins attenuate hepatic lipid accumulation through activating AMPK in human HepG2 cells

Chih-Li Lin, Hsiu-Chen Huang and Jen-Kun Lin¹

Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan 100

The online version of this article (available at http://www.jlr.org) contains supplementary data in the form of three figures.

Published, JLR Papers in Press, August 24, 2007.

¹ To whom correspondence should be addressed. e-mail: jklin{at}ha.mc.ntu.edu.tw

Black tea is one of the world's most popular beverages, and its health-promoting effects have been intensively investigated. The antiobesity and hypolipidemic effects of black tea have attracted increasing interest, but the mechanisms underlying these phenomena remain unclear. In the present study, the black tea major component theaflavins were assessed for their hepatic lipid-lowering potential when administered in fatty acid overload conditions both in cell culture and in an animal experimental model. We found that theaflavins significantly reduced lipid accumulation, suppressed fatty acid synthesis, and stimulated fatty acid oxidation. Furthermore, theaflavins also inhibited acetyl-coenzyme A carboxylase activities by stimulating AMP-activated protein kinase (AMPK) through the LKB1 and reactive oxygen species pathways. These observations support the idea that AMPK is a critical component of decreased hepatic lipid accumulation by theaflavin treatments. Our results show that theaflavins are bioavailable both in vitro and in vivo and may be active in the prevention of fatty liver and obesity.

Supplementary key words adenosine 5'-monophosphate-activated protein kinase • LKB1 • acetyl-coenzyme A carboxylase

Abbreviations: ACC, acetyl-coenzyme A carboxylase; AMPK, AMP-activated protein kinase; dsRNA, double-stranded RNA; HFD, high-fat diet; ROS, reactive oxygen species; siRNA, small interfering RNA

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