HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M700166-JLR200v1 48/11/2396    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bergeron, K. Articles by Thivierge, M. C. Search for Related Content PubMed PubMed Citation Articles by Bergeron, K. Articles by Thivierge, M. C. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 48, 2396-2410, November 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Long-chain n-3 fatty acids enhance neonatal insulin-regulated protein metabolism in piglets by differentially altering muscle lipid composition

Karen Bergeron^*,, Pierre Julien, Teresa A. Davis^**, Alexandre Myre^*, and M. Carole Thivierge^1,*,,

^* Department of Animal Science, Faculty of Food Sciences and Agriculture, Laval University, Québec, Québec G1K 7P4, Canada
Institute of Nutraceuticals and Functional Foods, Faculty of Food Sciences and Agriculture, Laval University, Québec, Québec G1K 7P4, Canada
Lipid Research Center, Laval University Hospital Center, Québec, Québec G1V 4G2, Canada
^** United States Department of Agriculture/Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, UK

Published, JLR Papers in Press, August 2, 2007.

¹ To whom correspondence should be addressed. e-mail: c.thivierge{at}rowett.ac.uk

This study investigated the role of long-chain n-3 polyunsaturated fatty acids (LCn-3PUFAs) of muscle phospholipids in the regulation of neonatal metabolism. Twenty-eight piglets were weaned at 2 days of age and raised on one of two milk formulas that consisted of either a control formula supplying 0% or a formula containing 3.5% LCn-3PUFAs until 10 or 28 days of age. There was a developmental decline in the insulin sensitivity of amino acid disposal in control pigs during the first month of life, with a slope of –2.24 µmol·kg^–1·h^–1 (P = 0.01) per unit of insulin increment, as assessed using hyperinsulinemic-euglycemic-euaminoacidemic clamps. LCn-3PUFA feeding blunted this developmental decline, resulting in differing insulin sensitivities (P < 0.001). When protein metabolism was assessed under parenteral feeding-induced hyperinsulinemia, LCn-3PUFAs reduced by 16% whole body oxidative losses of amino acids (from 238 to 231 µmol·kg^–1·h^–1; P = 0.06), allowing 41% more amino acids to accrete into body proteins (from 90 to 127 µmol·kg^–1·h^–1; P = 0.06). The fractional synthetic rate of muscle mixed proteins remained unaltered by the LCn-3PUFA feeding. However, LCn-3PUFAs retarded a developmental increase in the essential-to-nonessential amino acid ratio of the muscle intracellular free pool (P = 0.05). Overall, alterations in metabolism were concomitant with a preferential incorporation of LCn-3PUFAs into muscle total membrane phospholipids (P < 0.001), in contrast to intramuscular triglycerides. These results underscore the potential role of LCn-3PUFAs as regulators of different aspects of protein metabolism in the neonate.

Supplementary key words neonatal feeding • insulin sensitivity • phenylalanine kinetics • stable isotopes

Abbreviations: IE, isotopic enrichments; LCn-3PUFA, long-chain n-3 polyunsaturated fatty acid; PB, protein breakdown; PS, protein synthesis

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. C. Thivierge, J. A. Bush, A. Suryawan, H. V. Nguyen, R. A. Orellana, D. G. Burrin, F. Jahoor, and T. A. Davis Positive net movements of amino acids in the hindlimb after overnight food deprivation contribute to sustaining the elevated anabolism of neonatal pigs J Appl Physiol, December 1, 2008; 105(6): 1959 - 1966. [Abstract] [Full Text] [PDF]