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Originally published In Press as doi:10.1194/jlr.D700017-JLR200 on August 23, 2007

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Journal of Lipid Research, Vol. 48, 2514-2520, November 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Methods

A simple and rapid method to assay triacylglycerol in cells and tissues

Danielle M. Schwartz and Nathan E. Wolins1

Division of Nutritional Science, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110

Published, JLR Papers in Press, August 23, 2007.

1 To whom correspondence should be addressed. e-mail: nwolins{at}wustl.edu

We have developed a reliable, rapid, and economical assay for the quantification of triacylglycerol (TG) in cells and animal tissues. In a few hours, this assay quantifies microgram amounts of TG from tens or even hundreds of samples. The protocol includes an organic extraction to partition TG away from proteins and other hydrophilic molecules found in cells and tissues that may interfere with the colorimetric enzyme-linked TG detection method. In addition, this assay is economical, as no expensive reagents, supplies, or equipment are needed. Another benefit of this assay is that it does not require environmentally unfriendly halogenated solvents.

Supplementary key words fatty acids • fat • lipid • screen • acyl-glycerol

Abbreviations: IHW, isopropanol-hexane-water; LPL, lipoprotein lipase; TG, triacylglycerol; TO, Tet-On


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